骨载异烟肼缓释微球的制备及其在动物模型中的释放研究

目的：观察探讨骨载异烟肼聚乳酸微球的设计和制备方法及其在动物模型中的释放效果。方法：选择24只雄性新西兰大白兔作为实验动物模型，采用复乳法制备缓释微球，对微球进行形态学观察、粒径分布观察、体内释药和体外释药观察。结果：平均微球粒径（10.59±0.3）μm，平均包封率（44.9±0.9）%，平均载药率（13.0±0.6）%。在24h、72h、1w、3w微球组滑膜药物浓度明显高于口服组和原药组，P＜0.05。与口服组和原药组对比，微球组0～24h药物波动差值明显更低，P＜0.05。结论：本研究制备的骨载异烟肼缓释微球具有良好的缓释性，能够在抗结核治疗中置入骨结核病灶对术后病灶发挥持久的疗效。

Bone of Isoniazid Slow-release Microspheres Preparation and Its release in Animal Model Studies

Objective: to observe the investigate bone load isoniazid design and preparation methods of polylactic acid microspheres and its release effect in animal models. Methods:24 male New Zealand white rabbit as the experimental animal model, USES the compound emulsion of slow-release microspheres prepared, morphological observation, particle size distribution of microspheres and observation, the drug release in vivo and in vitro drug release, and so on. Results:the average microspheres particle size was (10.59 ±0.3)μm, the envelopment rate was (44.9 ±0.9)% on average, the average load rate was (13.0 ±0.6)%. Within 24h, 72h, 1w, 3w synovial drug concentration microspheres group was obviously higher than that of oral and technical group, P<0.05. Compared with oral and technical groups, drug microsphere group 0 to 24 h wave difference significantly lower, P<0.05. Conclusion:the preparation of bone institute take isoniazid slow-release autumn good slow-release, can put in anti-tuberculosis treatment of postoperative bone tuberculosis lesions lesions exert lasting curative effect.