Pan natural killer cell monoclonal antibodies and their relationship to the NK1.1 antigen |
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| Authors: | C L Sentman J Hackett T A Moore M M Tutt M Bennett V Kumar |
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| Affiliation: | Department of Pathology and Immunology Graduate Program, University of Texas Southwestern Medical Center, Dallas. |
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| Abstract: | The study of natural killer (NK) has been difficult because they account for a small percentage of peripheral blood and splenic lymphocytes and the paucity of NK specific antigens that have been identified. We have isolated pure populations of C57BL/6 (H-2b) NK cells using the IgG2b monoclonal antibody PK136 (anti-NK1.1). These NK1.1+ cells were used to immunize 129/J (H-2b) mice, and in this report, we describe three new NK specific monoclonal antibodies (SW3A4(IgM), SW4B12(IgG1), and SW2B4(IgG2b] and their relationship to the known murine NK antigen NK1.1. We have further characterized the NK1.1 antigen as a 39 kd molecule which is coded for by a gene which appears to map to chromosome 6. |
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