金樱根质量控制方法提升研究及等级评价体系的建立

目的 提升金樱根药材的质量控制方法,对其进行安全性分析与等级评价.方法 对现行质量标准进行总结,改进薄层鉴别方法,建立特征图谱,并对含量测定方法进行研究;采用电感耦合等离子体质谱法测定其铅、镉、砷、汞、铜的残留量;利用SPSS统计软件对其进行聚类分析.结果 TLC鉴别方法各斑点清晰,分离度良好,可以区分真伪,建立的特征...

Improvement of quality control method and establishment of grade evaluation system for Rose cymosa

Objective To improve the quality control method of Rose cymosa, and to carry out safety evalution and grade evaluation. Methods The statutory standard of Rose cymosa were summarized. The thin-layer chromatography(TLC)identification method was improved, the characteristic map was established, and the content determination method was studied.The residues of lead, cadmium, arsenic, mercury and copper in Rose cymosa were determined by inductively coupled plasma mass spectrometry(ICP-MS). Cluster analysis of Rose cymosa was carried out using SPSS statistical software. Results The spots on TLC plate were clear and well-separated, which can be used to identify the authenticity. The established characteristic spectrum identified 5 characteristic peaks and identified 1 components. The average content of lead, cadmium, arsenic and copper was 0.7, 0.1, 0.1, 5.0 mg/kg, respectively, and mercury was less than 0.01 mg/kg. The 30 batches of samples were divided into 3 categories, 1 batch of super grade, 9 batches of high quality and 20 batches of qualified. Conclusion The proposed methods are accurate, simple, and objective, and can be used for quality control of Rose cymosa. The content of heavy metals in Rose cymosa from different areas was all within the limit prescribed in China’s Green Thade Standards Importing & Exporting Medicinal plants & Preparation, which was at a safe level. This research proposes three ranks of medicinal materials specifications for different features to distinguish clearly, and easy to master in practice, which has certain guidance and practical significance for the collection, processing, sales, circulation and clinical application of medicinal materials.