健脾柔肝息风汤对抽动障碍模型大鼠脑组织神经元中DARPP-32、TH含量的干预作用

目的 研究健脾柔肝息风汤对抽动障碍(TD)模型大鼠皮质-纹状体-丘脑-皮质回路中多巴胺和环磷酸腺苷调节的磷蛋白(DARPP-32)、酪氨酸羟化酶(TH)含量的影响及其作用机制.方法 将40只SD大鼠随机分为空白组(10只),造模组(30只),造模成功后,将造模组随机分为模型组、氟哌啶醇组和健脾柔肝息风汤组,每组10只,...

Effect of Jianpi Rougan Xifeng Decoction on the content of DARPP-32 and TH in the brain neurons of model rats with tic disorder

Objective To study the effect of Jianpi Rougan Xifeng Decoction on the content of dopamine and adenosine3’5’-monophosphate-regulated phospho-protein(DARPP-32) and tyrosine hydrogenase(TH) in the cortex striatum-thalamus-cortex loop of model rats with tic disorder, and to explore its mechanism. Methods 40 SD rats were randomly divided into blank group(n=10) and modeling group(n=30), after successful modeling, the modeling group was randomly divided into model group, haloperidol group and Jianpi Rougan Xifeng Decoction group, with 10 rats in each group. 7 days after intraperitoneal injection, HE staining was used to observe the morphological structure of each area of cerebral cortex and the morphological changes of neurons, and immunohistochemical staining was used to detect the expression of DARPP-32 and TH. Results Compared with blank group, motor behavior score and rigid behavior score of model group increased, and the differences were statistically significant(P <0.05).Compared with model group, motor behavior score and rigid behavior score of Jianpi Rougan Xifeng Decoction group and haloperidol group were decreased, and the differences were statistically significant(P<0.05). Compared with blank group, the levels of DARPP-32 and TH in model group, haloperidol group and Jianpi Rogan Xifeng Decoction group decreased(P <0.05).Compared with model group, the levels of DARPP-32 and TH in haloperidol group and Jianpi Rogan Xifeng Decoction group increased, and their average optical density was significantly different(P <0.05). And there was no significant difference in the average optical density of DARPP-32 and TH between the haloperidol group and the Jianpi Rougan Xifeng Decoction group(P>0.05). Conclusion Jianpi Rougan Xifeng Decoction can increase the content of DARPP-32 and TH in brain tissues of rats with tic disorder, thus regulating the functional balance of dopaminergic nervous system and reducing the occurrence of tic symptoms.