藏药七味铁屑胶囊对CCL4大鼠肝纤维化程度的影响

目的观察藏药七味铁屑胶囊对CCL₄诱导的肝纤维化大鼠肝纤维化程度的影响。方法健康雄性SD大鼠60只,分为空白组(N组)、模型组(M组)、鳖甲软肝片组(Y组),以及七味铁屑胶囊高剂量组(QH组)、中剂量组(QM组)、低剂量组(QL组),每组10只。除N组外,其余大鼠建立大鼠CCL₄肝纤维化模型。造模4周后,测定肝脾指数;全自动生化仪检测血清天冬氨酸氨基转移酶(aspartateaminotransferase,AST)、丙氨酸氨基转移酶(alanineaminotransferase,ALT)含量;采用ELISA法检测肝组织肿瘤坏死因子-α(tumornecrosisfactor,TNF-α)、白细胞介素-1β(interleukin,IL-1β)水平;定量检测肝组织羟脯氨酸(hydroxyproline,HYP)含量和谷胱甘肽过氧化物酶(glutathioneperoxidase,GSH-Px)活力。结果与N组相比,M组肝脾指数明显升高(P<0.001);AST、ALT、HYP含量显著上升(P<0.001);TNF-α、IL-1β含量明显升高(P<0.05,P<0.01),GSH-Px活力显著降低(P<0.001)。与M组相比,各给药组肝脏指数均显著降低(P<0.01,P<0.001);QM组脾脏指数显著降低(P<0.01);Y组、QH组血清AST、ALT含量明显降低(P<0.05);Y组、QH组、QM组HYP含量显著降低(P<0.01,P<0.001),GSH-Px活力升高(P<0.01,P<0.001);Y组、QM组TNF-α水平明显降低(P<0.05),各治疗组IL-1β水平均显著下降(P<0.01,P<0.001);与Y组比,QH组GSH-Px活力明显升高(P<0.05)。结论藏药七味铁屑胶囊可减轻CCL₄诱导的大鼠肝纤维化程度,其机制可能与缓解炎症反应、抑制胶原生成、增强抗氧化能力有关。

Effect of Tibetan medicine Qiwei Tiexie Capsule on the degree of liver fibrosis rats induced by CCL4

Objective To observe the effect of Qiwei Tiexie Capsule on the degree of liver fibrosis in CCL₄-induced liver fibrosis rats.Methods 60 healthy male SD rats were divided into blank group (N group),model group (M group),Biejia Ruangan Tablet group (Y group),high-dose (QH),medium-dose (QM) and low-dose (QL) groups of Qiwei Tiexie Capsule,with 10 rats in each group.The CCL₄liver fibrosis model of rats was established except N group.After 4 weeks of modeling,the liver and spleen indexes were measured;the content of serum aspartate aminotransferase (AST) and alanine aminotransferase (ALT) was detected by automatic biochemical analyzer;the levels of tumor necrosis factor (TNF-α) and interleukin-1β(IL-1β) in liver tissues were detected by ELISA;the content of hydroxyproline (HYP) and the activity of glutathione peroxidase (GSH-Px) in liver tissues was determined quantitatively.Results Compared with the N group,the liver and spleen index of the M group increased significantly (P<0.001);the content of AST,ALT,and HYP increased significantly (P<0.001);the content of TNF-αand IL-1βincreased significantly (P<0.05,P<0.01);the activity of GSH-Px was significantly reduced (P<0.001).Compared with the M group,the liver index of each administration group significantly reduced (P<0.01,P<0.001);the spleen index of QM group significantly reduced (P<0.01);the content of serum AST and ALT of Y group and QH group significantly reduced (P<0.05);the content of HYP of Y group,QH group,QM group significantly reduced (P<0.01,P<0.001),the activity of GSH-Px significantly increased (P<0.01,P<0.001);the TNF-αlevel of liver tissue of Y group and QM group significantly reduced (P<0.05),the level of IL-1βdecreased significantly in all treatment groups (P<0.01,P<0.001);compared with the Y group,the activity of GSH-Px of the QH group was significantly increased (P<0.05).Conclusion Tibetan medicine Qiwei Tiexie Capsule can alleviate CCL₄-induced liver fibrosis in rats,which may be related with reduced inflammation,decreased collagen production,and enhanced antioxidant capacity.