五味子醋蒸前后药效差异比较研究

目的比较五味子生品及其醋蒸品不同提取物的主要药理作用差异。方法采用氢化可的松造阴虚小鼠模型,以免疫器官重量、单核细胞吞噬碳粒能力、血清溶菌酶含量及血浆环磷酸腺苷/环磷酸鸟苷(cAMP/cGMP)比值为指标,评价药物对阴虚小鼠免疫功能的作用;以大黄造小鼠脾虚模型,观察药物对肠炭末推进率的影响;采用浓氨水气雾致小鼠咳嗽模型,评价药物的止咳作用;建立氯化乙酰胆碱(Ach)诱导的豚鼠离体气管条收缩模型,检测药物对其收缩活性的影响。结果生、醋五味子对阴虚小鼠免疫功能均有一定的保护作用,对溶菌酶活性的影响,醋五味子水提物与生五味子水提物比较差异显著(P<0.01);醋五味子有显著的抑制小肠推进作用,以醋五味子混合物作用最强(P<0.01);生、醋五味子的醇提物与混合物均可减少浓氨水致小鼠咳嗽次数,生五味子混合物的抑制作用最强(P<0.01);生、醋五味子不同提取物均可显著抑制氯化乙酰胆碱诱导的豚鼠离体气管条收缩(P<0.05或P<0.01)。结论五味子醋蒸后,免疫功能保护和涩肠止泻的作用增强,但止咳与解痉的作用略弱。

Comparative Study on Effect Difference of Wuweizi(Schisandra Chinensis Fructus) before and after Vinegar Steaming

Objective To compare the main pharmacological effects of different extracts of Wuweizi(Schisandra Chinensis Fructus)(SCF) and its vinegar-steamed products. Methods The Yin deficiency mice model was established by hydrocortisone. The effects of herbs on the immune function of Yin deficiency mice were evaluated by taking the weight of immune organs, the ability of monocytes to phagocytize carbon particles, the content of serum lysozyme and the ratio of plasma cyclic adenosine monophosphate/cyclic guanosine monophosphate(cAMP/cGMP) as the indexes. The spleen deficiency model of mice was established by Dahuang(Radix Et Rhizoma Rhei), and the effect of herbs on the propulsion rate of intestinal charcoal was observed. The cough model of mice induced by concentrated ammonia aerosol was used to evaluate the relieving cough effect of the herb. The contraction model of isolated guinea pig tracheal strips induced by acetylcholine chloride was established to detect the effect of herbs on its contractile activity. Results Both raw and vinegar-steamed SCF had a protective effect on immune function of Yin deficiency mice, but the effect of water extract from vinegar-steamed SCF on lysozyme activity was stronger than that from raw SCF(P<0.01). Vinegar-steamed SCF had a significant inhibitory effect on small intestinal propulsion, and the mixed extract had the strongest inhibitory effect(P<0.01). The alcohol and mixture extract of raw and vinegar-steamed products could reduce the cough times caused by concentrated ammonia, and the inhibition effect of raw products mixture extract was the strongest(P<0.01). Different extracts of raw and vinegar-steamed products could significantly inhibit the contraction of isolated guinea pigs tracheal strips induced by Ach(P<0.05 or P<0.01). Conclusion Comparing with the raw SCF, the effects of vinegar-steamed products on immune function and anti-diarrhea were improved, but the effects of anti-tussive effect and spasmolysis were attenuated.