夹竹桃科DNA条形码的初步筛选

DNA条形码(DNA barcoding)是一种新的物种鉴定技术,COI序列已成功应用于动物的鉴定,但目前国际上还没有找到一个通用序列能鉴定所有植物物种。为探索适合于夹竹桃科植物DNA条形码序列,拟用matK、ITS2、ITS 3个常规引物序列对夹竹桃科5个属的部分植物进行基因序列对比分析,并运用MEGA 7.0构建系统进化树进行系统发育分析、鉴定其亲缘关系。结果显示在ITS2、ITS、matK 3个引物序列中,只有matK引物序列能正常扩增目的基因,扩增成功率达到100%,并且该引物序列对比分析和系统发育树的聚类结果与传统分类学的鉴定结果一致。建议将matK序列作为夹竹桃科DNA条形码鉴定候选序列之一。

Preliminary Selection of Potential DNA Barcoding for Apocynaceae

DNA barcoding is a new technique to identify species using DNA sequences from a small fragment of genomes. DNA barcoding, such as COI sequence, has been used to identify animal species. Unfortunately, there is no universally accepted barcode system applicable to plants. In order to find out a right DNA barcode regions in Apocynaceae, MEGA 7.0 was used to analyze the characters of sequences(matK.ITS2.ITS) and Taxon DNA was used to estimate the barcoding gap. The results showed that matK sequence could be taken as a potential barcode for identifying the species of Apocynaceae, and the amplification success rate reached 100%. DNA barcoding technology can identify species in Apocynaceae. And it is suggested to use matK sequence as one of the candidate sequences for the identification of Apocynaceae.