新型异黄酮对高糖诱导的白内障防治作用

目的 探讨新型异黄酮对高糖诱导的白内障防治作用.方法 8周龄雄性Wistar大鼠随机分为正常组(10只,给予0.3％羧甲基纤维素溶液),对照组(10只,给予10％高浓度果糖溶液),异黄酮低剂量组(9只,给予新型异黄酮1.0 mg·kg-1·d-1)和异黄酮高剂量组(9只,给予新型异黄酮10 mg·kg-1·d-1).采...

Effects of novel Isoflavones on prevention and treatment of high glucose-induced cataracts

Objective To investigate the effects of novel Isoflavones on the prevention and treatment of high glucose-induced cataracts.Methods Eight-week-old male Wistar rats were randomly divided into the normal group(n=10,given 0.3%Carboxymethyl cellulose),control group(n=10,given 10%high-concentration fructose),low-dose Isoflavones group(n=9,given novel Isoflavones 1.0 mg·kg^-1·d^-1)and high-dose Isoflavones group(n=9,given novel Isoflavones 10 mg·kg^-1·d^-1).The systolic and diastolic blood pressures were determined by blood pressure meter.The blood glucose was determined by blood glucose meter,and the vision was examined by ophthalmoscope.The morphological changes of the lens were recorded by photography.The rat lenses were taken,and the Ellman assay,hydrogen peroxide decomposition method,thiobarbituric acid reaction,colorimetric determination,and nitro blue tetrazole reduction method were used to measure the levels of reduced glutathione(GSH),lens catalase(CAT),lipid peroxidation(MDA),glutathione peroxidase(GSH-Px),and superoxide dismutase(SOD),respectively.Results Starting from the 4th week,the systolic blood pressure,diastolic blood pressure,and blood glucose levels in the control group significantly increased compared with those in the normal group(all P<0.05).Compared with the control group,the systolic blood pressure,diastolic blood pressure,and blood glucose levels significantly decreased in the low-dose and high-dose Isoflavones groups(all P<0.05).The 4 groups of rats developed cataracts at the end of 8 week.Compared with the control group,the incidence of stage 3 and 4 cataract formation was significantly reduced in the low-dose and high-dose Isoflavones groups,respectively(P<0.05,P<0.01).Compared with the control group,the lens opacity was significantly improved in the low-dose and high-dose Isoflavones groups(all P<0.05).The levels of GSH,GSH-Px,CAT,SOD and MDA in the low-dose and high-dose Isoflavones groups were significantly higher than those in the control group(all P<0.05).Conclusion The novel Isoflavones may prevent the formation of cataracts by reducing the lens oxidative stress in patients with hypertension and diabetes.