黄芩苷抑制乳腺癌细胞侵袭、迁移与凋亡的机制研究

目的 用黄芩苷干预人乳腺癌细胞株MCF-7,观察黄芩苷对乳腺癌细胞侵袭、迁移与凋亡的影响及其作用机制.方法 实验分为正常组、模型组、黄芩苷组、miR-126组、LNA组、LNA-126组和LNA-126+黄芩苷组.采用RT-PCR检测miR-126、miR-145、miR-100和let-7c的表达水平,MTT法检测细...

Mechanism of baicalin inhibiting invasion,migration and apoptosis of breast cancer cells

Objective Human breast cancer cell line MCF-7 was treated with baicalin to observe the effect and mechanism of baicalin on invasion, migration and apoptosis of breast cancer cells. Methods The experiment was divided into control group,model group, baicalin group, miR-126 group, LNA group, LNA-126 group and LNA-126+baicalin group. The expression levels of miR-126, miR-145, miR-100, let-7 c were detected by RT-PCR, and the cell proliferation was determined by using MTT method.The invasion and migration of cells were detected by Transwell test, and the expression levels of p53, Caspase-3, Caspase-9, Bcl-2,Bax and p-p38 were determined by Western blot. Cell apoptosis was detected by flow cytometry. Results The results of RT-PCR showed that compared with model group, the expression levels of miR-126, miR-145, miR-100, and let-7 c were significantly upregulated after baicalin was treated on MCF-7 cells(P <0.05,P <0.01,P <0.001), especially miR-126(P <0.001);compared with control group, the expression levels of four miRNAs in model group were down regulated, especially miR-126(P<0.05);MTT results showed that miR-126 and baicalin can inhibit the growth of MCF-7 cells(P<0.05), while LNA-126 may promote the growth MCF-7 cells(P<0.05). Transwell test results showed that miR-126 and baicalin can inhibit the invasion and migration of MCF-7 cells(P<0.05),while LNA-126 can antagonize the inhibitory effect of baicalin(P>0.05). Western blot results showed that the expression level of Bcl-2 decreased after baicalin and miR-126 acted on MCF-7 cells(P<0.05), and the expression levels of p53, Caspase-3, Caspase-9,Bax, and p-p38 increased(P<0.05). The results of flow cytometry showed that baicalin and miR-126 promoted the apoptosis of MCF-7 cells(P<0.05). Conclusion Baicalin can inhibit the proliferation, invasion and migration of breast cancer cells, and promote their apoptosis. The mechanism may be related to the up-regulation of miR-126.