氟伐他汀对人外周血T淋巴细胞活性的影响

 目的　研究氟伐他汀对人外周血T淋巴细胞活性的影响。方法　采用流式细胞术分析T细胞活化和增殖情况,ELISA法检测细胞因子分泌量,TransAM^TMELISA法测定活化T细胞核因子 (NFAT)的活化状态。结果　氟伐他汀能抑制T细胞表面CD69的表达,显著抑制T细胞增殖,对CD25的表达无影响。能抑制NFAT的活化,显著降低IL-2的产生,对IFN-γ和IL-10的分泌几无影响,但明显诱导IL-4的分泌。结论　氟伐他汀具有免疫抑制作用。

Effect of fluvastatin on the activity of human peripheral blood T lymphocytes

OBJECTIVE To study the inhibitory effect of fluvastatin on the activity of T lymphocytes.METHODS The activation and proliferation of T cells were examined by flow cytometric analysis. Cytokine secretion was determined by ELISA. TransAM^TM ELISA kit was used to evaluate the activation of nuclear factor of activated T lymphocytes (NFAT).RESULTS 5μmol·L^-1 fluvastatin caused a lower level of CD69 expression, accompanied by an essential suppression of T cells proliferation, whereas no modification in CD25 expression was found Also, fluvastatin strongly blocked NFAT activation, and reduced IL-2 secretion, while the level of secreted IL-10 and IFN-γ had no change, and the level of IL-4 even experienced a significant increase. CONCLUSION Fluvastatin had inhibitory effect on the activity of T lymphocytes.