骨宝液在去卵巢大鼠骨质疏松症中抑制骨转换作用的实验研究

目的：探讨骨宝液对去卵巢大鼠骨质疏松症中抑制骨转换作用。方法：60只大鼠去卵巢诱导骨质疏松症，随机分为正常对照组、模型组、尼尔雌醇组、骨宝液低、中、高剂量组。正常对照组、模型组以0．9％生理盐水每天10ml／kg，尼尔雌醇组以尼尔雌醇每2周1mg／kg，其他三组每天分别以骨宝液5、10、15ml／kg的剂量灌胃给药3个月后，以比色法测定其血清钙（s-Ca）、骨碱性磷酸酶（b-AKP）和血清抗酒石酸酸性磷酸酶（s-TRAP）的活性，竞争放射免疫法测定血清中骨钙素（s-BGP）的含量；双能x线骨密度仪（DEXA）测定股骨及腰椎的骨密度；三点弯曲试验法测定骨生物力学；扫描电镜进行骨小梁形态计量学分析。结果：骨宝液组与其他组比较，s-TRAP水平降低，腰椎骨密度增加，股骨抗弯抗拉能力改善，骨小梁形态改善，骨小梁平均宽度（MTT）与骨小梁面积百分比（TS％）显著增加。结论：骨宝液对去卵巢大鼠骨质疏松症具有防治作用，其机制可能与抑制骨转换有关。

The experimental study of the effects of Gubaoye(骨宝液)on osteoporosis in ovariectomized rats by inhibiting the bone turnover

Objective:To explore the effects of Gubaoye on osteoporosis in ovariectomized rats by inhibiting the bone turnover.Methods:Sixty rats were induced to osteoporosis by ovariectomy,and randomly divided into six groups(ten rats in each group):A control group,a model group,a Nilestriol group,a low-dose-Gubaoye group,a middle-Gubaoye group and a high-dose-Gubaoye group.The control and model groups were fed with 0.9% saline 10 ml/kg once a day.The Nlestriol group was fed with 1 mg/kg.The other three groups were fed with Gubaoye at the dose of 5,10,15 ml/kg once a day respectively.After fedding for 3 months,the concentration of calcium (s-Ca),tartrate resistant acid phosphatase in serum (s-TRAP)and alkaline phosphatase in bone (b-AKP)were measured by colorimetric method.The concentration of osteocalcin in serum (s-BGP)was measured by competitive radio immunologic method.Bone mineral density (BMD) of femur and lumbar of rats was determined by Dual Engergy X-ray Absorbtiometry (DEXA).Biomechanical characteristics of femur were measured by three point bending test.Histomorphometry of bone trabecula was analysed by scanning electron microscope.Results:As compared with the other groups,In the ubaoye groups,the level of s-TRAP was decreased;The bone density of lumbar vertebra was increased;The resistance of bending and compression of fumr of rats was enhanced;The form of bone trabecula was impoved and MTT and trabeculae square percentage (TS%) were obviously increased,as compared with other groups.Conclusion:Gubaoye has anti-osteoporotic effects on ovariectomized rats by inhibiting the bone turnover.