丙型肝炎患者血清HSP70水平的检测及HSP70-HCV肽诱导CTL作用

目的 :检测丙型肝炎患者及正常人血清中HSP70的水平 ,探讨HSP70 HCV肽体外诱导特异性CTL的作用。方法 :先用ELISA法筛选出丙型肝炎患者及正常人血清 ,再用双夹心ELISA法检测血清HSP70 ,并分析抗HCV抗体和HSP70之间的关系。用HSP70 HCV肽激活外周血单个核细胞后 ,通过51Cr释放试验测定CTL的杀伤活性。结果 :76份血清 (抗HCV抗体阳性 2 8例 ,正常人血清 4 8例 )中 ,抗HCV抗体阳性患者HSP70的检出率为 82 .1% ,正常人血清中HSP70的检出率为18.8%。HSP70的检出率与HCV感染呈显著相关 ( χ2 =2 8.77,P <0 .0 1)。HCV感染者血清HSP70的含量显著高于正常人。HSP70与HCVC区肽 (DLMGYIPAV)的混合物 ,可诱导 1例患者的CTL对自体HCV肽致敏靶细胞的杀伤率达 37.8% ,而用HCV肽单独作用则不显著。结论 :HCV感染可刺激机体过表达HSP70 ,同时HSP70可能有增强HCV表位肽递呈促进CTL特异性杀伤HCV感染细胞的作用

Detection of serum heat shock protein 70 level in patients with hepatitis C infection and its role in cytotoxicity of CTLs induced by HSP70-HCV peptide complex

AIM: To detect serum HSP70 in patients with hepatitis C and evaluate its role in cytotoxicity of specific CTLs induced by HSP70-HCV peptide complex. METHODS: The serum HSP70 level in patients infected with HCV and normal individuals were detected by ELISA. The relationship between anti-HCV antibody and HSP70 was evaluated. Peripheral blood mononuclear cells (PBMCs) were activated by HSP70-HCV peptide complex and then 4 hour (51)Cr release assay were used to detect the killer activity of CTLs. RESULTS: Detection rates of HSP70 were 82.1%(22/28) and 18.8% (9/48) in patients with anti-HCV antibody and in normal individuals, respectively. There was significant correlation between the detection rate of HSP70 and HCV infection (chi(2)=28.77, P<0.01). The level of serum HSP70 in patients infected with HCV was markedly more higher than that in normal individuals. CTLs from one patient induced by HSP70-HCV C region peptide (DLMGYIPAV) complex could lyse autologous B lymphoblastoid cell lines (BLcLs) with killer rate of 37.8%, whereas CTLs induced by peptide alone could not lyse autologous BLcLs cells. CONCLUSION: HCV infection can induce over expression of HSP70. HSP70 may have the action of enhancing presentation of HCV epitope peptide and promoting clearance cells infected with HCV.