Survivin在早期胃癌及癌前病变中的表达及其与PTEN表达的关系

目的：探讨Survivin在早期胃癌及癌前病变中的表达及其与PTEN表达的关系。方法：采用免疫组化SP法检测Survivin、PTEN基因蛋白在30例正常胃黏膜、20例不完全型大肠化生,93例轻、中、重度不典型增生和54例早期胃癌中的表达。结果：Survivin在正常胃黏膜不表达（0/30）,在不完全型大肠化生的阳性表达率为5.00%（1/20）;在轻、中和重度不典型增生的阳性率分别为6.25%（2/32）、8.57%（3/35）和65.73%（17/26）,其中轻、中度不典型增生与重度不典型增生的差异有统计学意义,P〈0.05。在早期胃癌中阳性表达率为68.51%（37/54）。Survivin基因在早期胃癌中的阳性与阴性表达中PTEN的阳性表达呈明显负相关,r=-0.564,P=0.0000。结论：Survivin在早期胃癌中表达上调,说明该基因在早期胃癌的发生过程中作用重要,Survivin表达越高,早期胃癌的分化程度越低。早期胃癌中Survivin的阳性表达与PTEN阳性表达密切相关。

Expression of Survivin protein and its relationship with expresion of PTEN protein in human early gastric cancer

OBJECTIVE:To explore the expression of Survivin in early gastric carcinoma and its relation with the expression of PTEN gene.METHODS:The immunohistochemical technique SP method was used to detect the expressions of Survivin and PTEN in 35 cases of normal gastric mucosa,20 cases of incomplete-type intestinal metaplasia,93 cases of mild,moderate and severe atypical nyperplasia,and 54 cases of early gastric carcinoma.RESULTS:The Survivin expression was not found in the normal gastric mucosa(0/30).The expression of Survivin gene was detected in incomplete-type intestinal metaplasia(5.00%,1/20).The positive rates of Survivin expression were 6.25%(2/32),8.57%(3/35),65.73%(17/26)in mild,moderate,severe atypical nyperplasia,respectively,and 68.51%(37/54)in early gastric carcinoma.The expresion rate of Survivin was negatively correlated with that of PTEN protein r=-0.564,P=0.0000.CONCLUSION:The abnormal expression of Survivin might play an important role in the onset and progression of early gastric carcinoma and there is a potential correlation between Survivin and PTEN expressions.