地塞米松防治豚鼠钩端螺旋体病肺弥漫性出血机理的实验研究

为探讨地塞米松对豚鼠钩端螺旋体病肺弥漫性出血（ＰＤＨ）的治疗机理，将豚鼠随机分为感染钩端螺旋体肺弥漫性出血组、实验治疗组、地塞米松对照组和正常对照组，每组各１０只。观察４ 组豚鼠的大体解剖，肺、肝、肾组织结构和肝线粒体的超微结构，并测定其肝脏乳酸和线粒体ＡＴＰａｓｅ活性的变化。结果显示： 实验治疗组的上述各项观察指标与其余３ 组比较均有显著性差异（Ｐ＜ ０．０１）。由此提示早期静脉注入５ｍ ｇ 地塞米松，对豚鼠ＰＤＨ 可能有一定的防治作用，其机制可能与地塞米松可稳定线粒体膜，抑制肝乳酸含量，促进线粒体ＡＴＰ酶活性密切相关。

Experimental Study of the Effect of Dexamethasone on Pulmonary Diffuse Hemorrhage and Energy Metabolism of Mitochondria in the Liver of Guinea Pig Infected with Leptospirosis

This experiment was designed to study the curative effect of dexamethasone on leptospirosis with pulmonary diffuse hemorrhage(PDH) in guinea pig model.The guinea pigs were randomly divided into four groups:the infected group,all animals developed PDH( n =10);the infected plus treated group( n =10);the treated control group( n =10);and the normal control group( n =10).The gross and microscopic observations in lung, liver and kidney were made. Also, ultrastructural changes of mitochondria in liver were examined,and the lactic acid level and mitochondrial ATPase activity in liver were determined.The results showed that there was significant difference in mortality,in gross and microscopic changes in lung,liver and kidney,and in the changes of mitochondrial ultrastructures,ATPase activity and lactic acid level in liver between the infected group and the infected plus treated group( P <0.01).These findings suggest that the pharmacologic dose of dexamethasone injected intravenously in the early stage of PDH may have some beneficial effect on the emergency treatment of leptospirosis with PDH. The mechanism appears to be related to its effect on the inhibition of glycolysis and increasing oxidative phosphorylation in mitochondria.