A method for the isolation of cells from arteries of various sizes

Summary This report describes a technique for the isolation of smooth muscle cells (SMC) from arteries of different sizes. SMC from dog carotid arteries were dissociated by collagenase-elastase perfusion under constant pressure. The freshly isolated cells were spindle shaped with an average length of 118.0 + 8.0 μm. A distinct basal lamina, ovoid nucleus, and both thick and thin filaments were observable by transmission electron microscopy. Scanning electron microscopy of the cells revealed a continuous plasma membrane and several fingerlike processes. A chamber used to study the contractile properties of isolated cells is also described. Studies of the contractile properties of SMC isolated by this technique found that cells exposed to calcium (2.5 mM) or potassium (50 mM) or both contracted by 25 to 35%. Thus, SMC isolated by the collagenase-elastase perfusion technique described here possess morphologic and contractile characteristics typical of SMC. This research was supported by Veterans Administration medical research funds.