A Simple Method to Calculate Resolving Power and Confidence Interval of QTL Map Location |
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Authors: | A. Darvasi M. Soller |
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Affiliation: | (1) Department of Genetics, The Alexander Silberman Life Sciences Institute, The Hebrew University of Jerusalem, 91904 Jerusalem, Israel;(2) The Jackson Laboratory, Bar Harbor, Maine, 04609-1500 |
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Abstract: | Resolving power is defined as the 95% confidence interval for quantitative trait locus (QTL) map location that would be obtained when scoring an infinite number of markers in a given constellation of a marker-QTL mapping experiment. Resolving power can serve as a close estimate of the confidence interval of QTL map location, as well as a guide to the lower efficient limit of marker spacing in an initial marker-QTL mapping experiment. In the present study, an extensive series of simulations was carried out to provide estimates of resolving power, for backcross (BC) and F2 designs, over a wide range of experimental sizes and of gene effects and dominance at the QTL. From the simulation results, the remarkably simple expressions, 3000/(mNd2) (where m = 1 for BC and m = 2 for F2; N = population size, and d = allele substitution effect) and 530/N (in terms of , the proportion of variance explained), were obtained for estimating resolving power. These expressions can provide a convenient guide to planning marker spacing in BC and F2 marker-QTL linkage experiments and for placing confidence intervals about QTL map location obtained in such experiments. |
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Keywords: | Quantitative trait loci (QTL) confidence interval resolving power gene mapping |
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