CD81和LDLR在不同妊娠时期绒毛组织中的定位与表达

目的检测CD81、低密度脂蛋白受体(LDLR)在人胎盘绒毛组织滋养层细胞中mRNA表达水平,了解一些可能参与丙型肝炎病毒(HCV)入胞的宿主因子表达。方法通过逆转录聚合酶链反应(RT-PCR)检测CD81、LDLR在人胎盘绒毛组织滋养层细胞中mRNA表达水平,并采用免疫荧光方法检测CD81、LDLR在不同孕期胎盘绒毛组织的表达。结果发现不同孕期胎盘绒毛组织的CD81、LDLR表达量随孕期呈递增趋势。采用免疫荧光方法证实了CD81、LDLR在不同孕期胎盘绒毛组织的表达。结论胎盘绒毛组织滋养层细胞中可以定位并表达HCV入胞的宿主相关因子CD81、LDLR。为进一步研究CLEC4M传播HCV的分子机制奠定了实验基础。

Localization and expression of CD81 and LDLR in placental villi during different stages of pregnancy

Objective To detect the expression of CD81 and low density lipoprotein receptor(LDLR) mRNA in cultured human trophoblast cells from different placental villi,and to explore HCV entry mechanisms.Methods CD81 and LDLR mRNA expression in trophoblast cells from human placental villi was detected by RT-PCR,and expression of CD81 and LDLR was also detected during different pregnancy stages by immunofluorescene method.Results CD 81 and LDLR were found in different placental villi and their expression has shown an increasing trend during different stages of gestation by immunofluorescene.Conclusion The localization and expressions of HCV entry-related receptor CD 81 and LDLR are found in cultured human trophoblast cells,which lay the foundation for further exploring the molecular mechanism of HCV infection transmission by CLEC4M.