| 糖基化终产物对人单核细胞EMMPRIN基因表达的影响 |
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| 引用本文： | 孙晔萍,孙子林,周莉. 糖基化终产物对人单核细胞EMMPRIN基因表达的影响[J]. 南通医学院学报, 2009, 29(2) |
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| 作者姓名： | 孙晔萍 孙子林 周莉 |
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| 作者单位： | 1. 江苏省靖江市人民医院内科,靖江,214500
2. 东南大学临床医学院,南京,210009 |
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| 摘 要： | 目的:探讨糖基化终产物(AGEs)对人单核细胞细胞外基质金属蛋白酶诱导因子(EMMPRIN)表达的影响。方法:在培养的人单核细胞株中分别加入不同浓度AGEs干预24h和同一浓度AGEs干预不同的时间点,以1640培养基和相应浓度的牛血清白蛋白为对照组。用逆转录聚合酶链反应检测EMMPRIN mRNA的表达水平。结果:AGEs干预人单核细胞的EMMPRIN mRNA水平与对照组相比差异有统计学意义(P<0.05);且其表达呈时间和浓度依赖性降低(P<0.05)。结论:AGEs以时间及浓度依赖的方式减少人单核细胞EMMPRIN mRNA的表达。
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| 关 键 词： | 糖基化终产物 人单核细胞 细胞外基质金属蛋白酶诱导因子 逆转录聚合酶链反应 糖尿病 |
Effect of advanced glycation end products on the gene expression of EMMPRIN in cultured human monocytes |
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| SUN Yeping,SUN Zilin,ZHOU Li. Effect of advanced glycation end products on the gene expression of EMMPRIN in cultured human monocytes[J]. ACTA Academiae Medicinae Nantong, 2009, 29(2) |
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| Authors: | SUN Yeping SUN Zilin ZHOU Li |
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| Affiliation: | SUN Yeping,1SUN Zilin,1ZHOU Li |
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| Abstract: | Objective:To investigate the effects of advanced glycosylation end products (AGEs) on the expression of extra- cellular matrix metalloproteinase inducer(EMMPRIN) in cultured human monocytes. Methods:The cultured human monocytes were incubated with AGEs (50,100,200,400 mg·L-1) for 24 h or 200 mg·L-1 AGEs for 8、16、24 and 48 h respectively,which were incubated with 1640 medium and BSA as a control. The mRNA expression of EMMPRIN in human monocytes was ana- lyzed by RT-PCR. Results:Compared with that in cells i... |
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