| LC－MS/MS法测定升麻葛根汤颗粒在犬体内的药代动力学研究 |
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| 引用本文： | 常梦春,刘静静,刘勃缨,任静,张丽娜,李佳玮,刘志东.LC－MS/MS法测定升麻葛根汤颗粒在犬体内的药代动力学研究[J].天津中医药大学学报,2019,38(1):68-73. |
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| 作者姓名： | 常梦春 刘静静 刘勃缨 任静 张丽娜 李佳玮 刘志东 |
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| 作者单位： | 天津中医药大学现代中药发现与制剂技术教育部工程中心;天津中医药大学天津市现代中药重点实验室-省部共建国家重点实验室培育基地 |
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| 基金项目： | 天津市自然科学基金（15PTCYSY00030，16ZXHLGX00170）。 |
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| 摘 要： | 目的]建立液相色谱-质谱联用(LC-MS/MS)的方法测定犬灌胃给药升麻葛根汤颗粒后葛根素、芍药苷、异阿魏酸的药代动力学。方法]采用Waters ACQUITY UPLCBEH C18柱(2.1 mm×50 mm,1.7μm),流动相:0.05%甲酸水(A)-乙腈(B)梯度洗脱;流速:0.2 mL/min;柱温:25℃;进样量:5μL。以甲苯磺丁脲为内标,采用电喷雾离子源(ESI离子源),在负离子检测方式下进行多反应离子检测(MRM),检测离子对分别为m/z 415.2→294.9(葛根素)、m/z 525.0→449.0(芍药苷)、m/z 193.05→133.9(异阿魏酸)、m/z 269.09→169.9(甲苯磺丁脲,内标)。结果]葛根素、芍药苷、异阿魏酸在10～4 000 ng/mL范围内线性关系良好。血浆中内源性物质不干扰测定,方法回收率、基质效应、精密度、准确度和稳定性均符合生物样品的测定要求。结论]该方法准确度强,专属性好,灵敏度高,可用于检测葛根素、芍药苷、异阿魏酸的药代动力学。
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| 关 键 词： | 升麻葛根汤颗粒 LC－MS/MS 犬 药代动力学 |
| 收稿时间： | 2018/10/25 0:00:00 |
Study on the pharmacokinetics of Shengma Gegen decoction granule in beagle dogs by LC-MS/MS |
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| CHANG Mengchun,LIU Jingjing,LIU Boying,REN jing,ZHANG Lin,LI Jiawei and LIU Zhidong.Study on the pharmacokinetics of Shengma Gegen decoction granule in beagle dogs by LC-MS/MS[J].Journal of Tianjin University of Traditonal Chinese Medicine,2019,38(1):68-73. |
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| Authors: | CHANG Mengchun LIU Jingjing LIU Boying REN jing ZHANG Lin LI Jiawei and LIU Zhidong |
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| Institution: | Engineering Research Center of Modern Chinese Medicine Discovery and Preparation Technique, Ministry of Education, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China;Tianjin State Key Laboratory of Modern Chinese Medicine, Tianjin 300193, China,Engineering Research Center of Modern Chinese Medicine Discovery and Preparation Technique, Ministry of Education, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China;Tianjin State Key Laboratory of Modern Chinese Medicine, Tianjin 300193, China,Engineering Research Center of Modern Chinese Medicine Discovery and Preparation Technique, Ministry of Education, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China;Tianjin State Key Laboratory of Modern Chinese Medicine, Tianjin 300193, China,Engineering Research Center of Modern Chinese Medicine Discovery and Preparation Technique, Ministry of Education, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China;Tianjin State Key Laboratory of Modern Chinese Medicine, Tianjin 300193, China,Engineering Research Center of Modern Chinese Medicine Discovery and Preparation Technique, Ministry of Education, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China;Tianjin State Key Laboratory of Modern Chinese Medicine, Tianjin 300193, China,Engineering Research Center of Modern Chinese Medicine Discovery and Preparation Technique, Ministry of Education, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China;Tianjin State Key Laboratory of Modern Chinese Medicine, Tianjin 300193, China and Engineering Research Center of Modern Chinese Medicine Discovery and Preparation Technique, Ministry of Education, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China;Tianjin State Key Laboratory of Modern Chinese Medicine, Tianjin 300193, China |
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| Abstract: | |
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| Keywords: | Shengma Gegen decoction granule LC-MS/MS beagle dogs pharmacokinetics |
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