| 双轴旋转运动刺激后大鼠中缝大核神经元活动分析 |
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| 引用本文： | 方岩,张富兴,陈琴,张浩强,张勇,冯宇鹏. 双轴旋转运动刺激后大鼠中缝大核神经元活动分析[J]. 神经解剖学杂志, 2017, 0(6): 659-664. DOI: 10.16557/j.cnki.1000-7547.2017.06.003 |
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| 作者姓名： | 方岩 张富兴 陈琴 张浩强 张勇 冯宇鹏 |
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| 作者单位： | 1. 第四军医大学人体解剖学教研室暨梁銶琚脑研究中心,西安,710032;2. 第四军医大学放射医学教研室,西安,710032;3. 兰州军区总医院骨科,兰州,730050 |
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| 基金项目： | 国家自然科学基金(81171279;81071105),陕西省社会发展科技攻关项目(2016SF119) |
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| 摘 要： | 目的:分析大鼠经引发晕动病的双轴旋转运动刺激后,中缝背核(DR)内5-HT能神经元激活状况,以探讨5-HT水平与前庭刺激的关系.方法:将雄性SD大鼠随机分成两组:对照组和双轴旋转运动刺激组.所有动物装入定做的有机玻璃圆筒内,并于黑暗中进行如下处理2h:运动刺激组动物以电动马达驱动的双轴旋转运动刺激;对照组动物放置在距刺激仪器20 cm的位置.应用包含DR的冠状脑切片,以双重免疫荧光标记技术标记5-HT和Fos蛋白,并对标记神经元进行计数和统计分析.结果:对照组和旋转运动刺激组的Fos阳性神经元、5-HT阳性神经元以及Fos/5-HT双标记神经元的数目分别是:93.4 ±12.0 vs 153.1 ±21.1、528.5±36.0vs 531.1±23.4和15.1 ±11.3 vs 30.8 ±11.3.t检验分析显示两组Fos阳性神经元数目有显著性差异(P＜0.05);Fos/5-HT双标神经元数目在旋转运动刺激后有增加趋势.结论:引发晕动病的双轴旋转运动能激活中缝背核内的神经元,其中的5-HT能神经元也对刺激有反应.
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| 关 键 词： | 晕动病 5-HT 前庭系统 中缝背核 大鼠 |
Analysis of neuronal activation in dorsal raphe nucleus of rats following stimulation with double-axis rotation |
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| Fang Yan,Zhang Fuxing,Chen Qin,Zhang Haoqiang,Zhang Yong,Feng Yupeng. Analysis of neuronal activation in dorsal raphe nucleus of rats following stimulation with double-axis rotation[J]. Chinese Journal of Neuroanatomy, 2017, 0(6): 659-664. DOI: 10.16557/j.cnki.1000-7547.2017.06.003 |
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| Authors: | Fang Yan Zhang Fuxing Chen Qin Zhang Haoqiang Zhang Yong Feng Yupeng |
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| Abstract: | Objective:To evaluate the serotonergic neuron activation in dorsal raphe nucleus (DR) of rat upon ex posure to double-axis rotation stimulation which can evoke motion sickness;thus to explore the correlation of 5-HT and vestibular stimulation.Methods:Male Sprague-Dawley rats were randomly divided into two groups:control and doubleaxis rotation groups.All animals were singly restrained in custom-made Perspex containers and were subjected to either of the following treatments for 2 hours in darkness:motor-driven double-axis rotation for rotation group or staying in place 20 cm away from the rotating equipment for the control group.Double immunofluorescence for 5-HT/Fos was per formed on coronal brain sections containing dorsal raphe,and the labeled neurons were counted and statistically analyzed.Results:The number of Fos-positive,5-HT-labeled and Fos/5-HT double labeled neurons in DR for control vs experimental groups were counted to be 93.4 ± 12.0 vs 153.1 ± 21.1,528.5 ± 36.0 vs 531.1 ± 23.4 and 15.1 ± 11.3 vs 30.8 ± 11.3 per section,respectively.Statistical analysis with t-test showed that Fos-labeled neurons between two groups showed a significant difference (P ＜ 0.05);while Fos/5-HT double-labeled neurons showed an obvious trend to increase in number following rotation stimulation.Conclusion:Double-axis rotation which induces motion sickness can activate dorsal raphe nucleus neurons,among which 5-HTergic neurons may also respond to the stimulation. |
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| Keywords: | motion sickness 5-HT vestibular system dorsal raphe nucleus rat |
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