多重PCR在幽门螺杆菌检测及分型中的应用 |
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引用本文: | 杨学文,王礼文.多重PCR在幽门螺杆菌检测及分型中的应用[J].临床检验杂志,2000,18(3):148-150. |
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作者姓名: | 杨学文 王礼文 |
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作者单位: | 江苏省中医院,南京,210029 |
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摘 要: | 应用多重PCR同时扩增16SrRNA和cagA基因来检测幽门螺杆菌感染及其分型。PCR产物经DNA序列测定证实为转异性扩增,敏感度为10^2CFU/ml。48株Hp菌株中,I型菌株(cagA+)26株(54.2%);550份胃粘液标本,Hp阳性(16SrRNA基因+)216份(47.5%),其中I型Hp139份(53.3%)。
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关 键 词: | 幽门螺杆菌 多重PCR 16SrRNA基因 cagA |
Use of multiplex PCR for detection and typing of Helicobacter pylori |
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Abstract: | A multiplex PCR assay with simultaneous amplification of 16 SrRNA and cagA gene was developed for detection and typing of Helicobacter pylori(Hp).PCR products were confirmed to be special amplification by DNA sequence.The sensitivity was 10 2 CFU/ml.Among 48 Hp strains,26(54 2%)were type I Hp strains (cagA ).Of 550 gastric secretion samples,261(47 5%)were Hp positive(16 SrRNA gene )and 139(53 3%)were type I. |
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Keywords: | Helicobacter pylori multiplex PCR 16 SrRNA gene cytotoxin associated gene A |
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