注射用三七提取物的纯化方法优选

目的优选注射用三七提取物的纯化方法。方法以三七总皂苷的转移率、质量分数、杂质的清除效果和复溶性作为考察指标,采用明胶沉淀法、澄清剂法、石硫醇法、碱性醇沉法和大孔吸附树脂法对三七进行纯化,优选纯化条件,并对纯化结果进行比较。结果5种纯化方法单独使用均不能达到注射用要求,将碱性醇沉法和大孔吸附树脂法联用纯化三七药材,三七总皂苷的转移率和质量分数较高,杂质清除效果较好,复溶性较好。结论通过对不同纯化方法的比较,碱性醇沉法和大孔吸附树脂法联用可提高三七的纯化效果,并能达到注射用要求。

Optimization of purification of Panax notoginseng extract for injection

Objective To optimize the purification method of Panax notoginseng extract for injection.Methods Taking the transfer rate,quality point of Panax notoginsenoside(PNS),scavenging effect of impurities,and redissolubility as the estimated targets,optimizing the effects of glutin precipitation,ZTC1 1 clarifying agent,lime creamsulphuric alcohol,basic alcohol precipitation,and macroporous resin adsorption methods,and then comparing with the effects of purification of P.notoginseng.Results The five purification methods used alone could not achieve the injection standard.When using basic precipitation and macroreticular resin adsorption method to purify P.notoginseng,the transfer rate and quality point of PNS were higher.Both the scavenging effect of impurities and redissolubility were better.Conclusion By the comparison of different purification methods, it is found that the method using basic precipitation and macroporous resin adsorption could improve the purification,and achieve the standard for injection.