人胚胎胰岛分泌白细胞介素6的动力学研究

目的：阐述ＩＬ－６与胰岛功能或胰岛病变的可能关系。方法：采用常规消化方法分离人胚胎胰岛并进行原代培养，培养上清进行ＩＬ－６、胰岛素、胰高血糖素活性测定和ＩＬ－６ＭｃＡｂ中和实验，同时对胚胎胰岛进行体外葡萄糖刺激实现。结果：原代培养的胚胎胰岛在４０ｈ内ＩＬ－６分泌呈上升趋势（３７～１５３ｍＵ／胰岛），每２４ｈ重新换取新培养液作为一个分泌周期，ＩＬ－６分泌以第１周期最高（１０６ｍＵ／胰岛），体外连续培养ＩＬ－６的分泌以１ｄ最高（１４５ｍＵ／胰岛），用ＩＬ－６ＭｃＡｂ可以阻断胰岛培养上清中ＩＬ－６的活性；胰岛素、胰高血糖素的分泌周期与ＩＬ－６不同；葡萄糖刺激明显促进ＩＬ－６的分泌，其分泌趋势与胰岛素分泌一致。结论：原代培养的人胚胎胰岛在无任何刺激情况下可自发分泌ＩＬ－６，而且ＩＬ－６的分泌有自己的分泌环路。

IL-6 secretion kinetics of human fetal islets

AIM:To study the relationship between IL-6 and function of pancreatic islets, IL-6 secretion kinetics of hmuan fetal pancreatic islets was investigated.METHODS:Islets from fetal pancreas were separated by collagenase type V (0 5 g/L) and cultured in vitro. The supernatants of primary culture of hmuan fetal islets for different days were assayed for IL-6, insulin and glucagon. The islets were stimulated by glucose and the supernatants was assayed too.RESULTS:IL-6 content of islet supernatant increased and peaked at 40 hour after stimulation (from 37 mU/islet to 153 mU/islet). Islets were replenished every 24 hours and secretion in first 24 h was highest (106 mU/islet). Cumulative Il-6 content in culture was highest on day 1 (145 mU/islet). IL-6 secretion was promoted dose-dependently after adding glucose to islets culture and insulin secretion was paralleled to IL-6 production.CONCLUSION:Islets may secret IL-6 spontaneously, and the glucose stimulation may regulate the IL-6 secretion, which is related to insulin production.