原发性肝癌中Bax及c-myc蛋白表达与凋亡指数的关系

目的　检测Bcl- 2、Bax及c -myc在肝细胞癌中的表达 ,探讨肝细胞癌中二者表达与凋亡指数的关系。方法　对 2 2例人肝细胞癌及癌旁组织分别进行Bax及c -myc的免疫组织化学研究和TUNEL技术标记 ,计数阳性细胞数。结果　在肝细胞癌中Bax蛋白呈低表达 ,表达率为 45 45 % ,与癌旁组织相比有显著差异 (P <0 0 1) ;c -myc在肝细胞癌及癌旁组织中均呈高表达 ,表达率分别为 81 82 %和 72 73% ,但二者无差异 (P >0 0 5 ) ;肝癌组织中凋亡指数为 9 5 5 % ,低于癌旁组织15 6 8% ,二者有显著差异 (P <0 0 1)。结论　肝细胞癌的发生与细胞凋亡受到抑制有关 ,Bax起到更关键的作用 ;Bcl- 2 /Bax异二聚体能更准确地反映凋亡发生的程度 ;凋亡指数有可能成为判定细胞癌变的指标。

Relationship between Bax and c - myc and apoptosis index in human hepatocellular carcinoma

Objective To investigate the expression of Bax and c-myc in human hepatoellular carcinoma and paracancerous tissues.To evaluate the relationship between Bax and c-myc apoptosis index in human hepatocellular carcinoma.Method We observed the expression of Bax,c-myc and apoptosis index using immunohistochemical staining and terminal deoxynuleotidyl transferase nick and labelling technique in 22 human hepatocellular carcinoma and paracancerous tissues.Result The rate of Bax protein positive expression in hepatocellular carinoma was 45 45% and it was 77 27% in paracancerous tissues.This was statisticant as compared with each other ( P <0 01) The rate of c-myc protein expression in hepatocellular carcinoma was higher than that in paracancerous tissues,being 81 82% and 72 73%,respectively.But no difference was found.The apoptosis index in hepatocellular carcinoma was 9 55% and was lower than that in paracancerous tissues( P <0 01).Conclusions There were relationship between the occurrence of hepatocellular carcinoma and the apotosis.Bcl-2/Bax could reflect the degree of apoptosis accurately.Bax protein play an important role in the occurence of hepatocellular carcinoma.The apoptosis index would become an croial value to evaluate malignancy change of cells [