A rosette assay for identification of Ia-like alloantigens on chicken lymphoid cells |
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| Authors: | Jerzy S. Nowak Olli Vainio Olli Lassila Paavo Toivanen Douglas G. Gilmour |
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| Affiliation: | 1. Department of Medical Microbiology, Turku University, Turku, Finland;2. Basel Institute for Immunology, Basel, Switzerland;3. Department of Microbiology, New York University School of Medicine, New York, NY, U.S.A. |
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| Abstract: | An Ia-rosette assay for the detection of Ia-like alloantigens on the chicken lymphoid cell surface is described. The method is based on the ability of cells treated with allo-antiserum to Ia and then with rabbit antiserum against the Fc portion of chicken IgG, to form rosettes with sheep erythrocytes (SRBC) coated with chicken anti-SRBC IgG antibody. Interference with Ia-rosette formation by Fc IgG receptors was eliminated by pronase treatment which removes Fc receptor activity without affecting Ia antigens. The Ia-rosette assay is at least as sensitive as the triple-layer immunofluorescence test for identification of Ia antigens. In addition, the assay allows morphological study and separation of the rosetting cells. |
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| Keywords: | Address correspondence to: Dr. Paavo Toivanen Department of Medical Microbiology Turku University SF-20520 Turku 52 Finland. |
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