An improved method for the isolation of the major protein of the gonococcal outer membrane in an antigenically reactive form |
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Authors: | L.T. James J.E. Heckels |
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Affiliation: | Department of Microbiology, Southampton University Medical School, Southampton General Hospital, Tremona Road, Southampton SO9 4XY, U.K. |
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Abstract: | The major outer membrane protein (protein I) has been isolated from Neisseria gonorrhoeae strain P9 in an immunologically reactive form. Membranes were sequentially extracted with the detergents sodium cholate and Empigen BB. Protein I was enriched in the Empigen-soluble fraction and was separated from other proteins and lipopolysaccharide by gel filtration chromatography on Sephadex G-200. The purified protein retained its antigenic activity with antiserum raised against the unfractionated outer membrane complex. |
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Keywords: | Address all reprint requests to B.E. Samuelsson University of Göteborg Department of Medical Biochemistry Box 330 31 S-400 33 Göteborg Sweden. |
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