妊娠期肝内胆汁淤积症脐血淋巴细胞凋亡的初步研究

目的 探讨脐血淋巴细胞凋亡在妊娠期肝内胆汁淤积症(intrahepatic cholestasis of pregnancy,ICP)发病中的意义.方法 采用流式细胞技术分析ICP患者和正常妊娠妇女脐血淋巴细胞的凋亡率;通过免疫细胞化学染色技术检测ICP患者和正常妊娠妇女脐血淋巴细胞上凋亡调控基因Fas、FasL、Bcl-2的蛋白表达.结果 ICP患者脐血淋巴细胞凋亡率低于正常妊娠组(P＜0.001),脐血淋巴细胞上Fas、Bcl-2表达水平两组间差异无统计学意义(P＞0.05),而FasL表达水平在ICP组高于正常妊娠组(P＜0.005).结论 ICP患者存在脐血淋巴细胞凋亡率及凋亡调控基因表达的异常,可能致移植物抗宿主反应异常而在ICP的发病中起一定作用.

The Prebiminary Study on Cord Blood Lymphocytes Apoptosis in Patients with Intrahepatic Cholestasis of Pregnancy

Objective Intrahepatic cholestasis of pregnancy (ICP) is a disease happening during pregnancy and does a great harm to the fetus. The etiology and pathogenesis of ICP are not clear. It is very important to investigate the relationship between ICP cord blood lymphocytes (CBLs) apoptosis and its clinical significance. Methods The CBLs were isolated from 20 patients with ICP and 20 normal pregnant women. Flow cytometry (FCM) was used to detect the apoptosis cells in the CBLs. The apoptosis regulatory molecules (Fas/FasL, Bcl-2) of CBLs were detected by immunohistochemical technique. Results The percentage of apoptotic CBLs in ICP patients were significantly lower than that in the normal pregnant women (P<0.05). The FasL protein expression of CBLs in ICP patients was significantly higher than that in normal pregnant women (P<0.05), but Fas and Bcl-2 protein expression were normal. Conclusion The abnormal apoptosis of CBLs may influence the immune reaction of ICP patients, which does something in the pathogenesis of ICP.