基质金属蛋白酶-9及其抑制因子在肾细胞癌组织中的表达和意义

目的　探讨基质金属蛋白酶 9(MMP 9)和金属蛋白酶组织抑制因子 1(TIMP 1)在肾细胞癌中的表达及其与临床病理参数之间的关系。方法　采用免疫组织化学链霉菌抗生物素蛋白过氧化酶 (SP)法检测 5 5例肾细胞癌中MMP 9和TIMP 1的表达情况。结果　MMP 9、TIMP 1蛋白在肾细胞癌和正常肾组织中的阳性表达率各为 63 .63 %和 10 .0 0 % (P <0 .0 5 ) ;60 .0 0 %和10 .0 0 % (P <0 .0 5 )。在肾癌中 ,MMP 9蛋白表达与肿瘤Robson分期、肾包膜侵袭和淋巴结转移密切相关 (P <0 .0 5 ) ,而与组织学类型无关 (P >0 .0 5 ) ;TIMP 1蛋白表达与肾细胞癌的临床病理参数无关 (P >0 .0 5 )。结论　MMP 9蛋白高表达参与了肾癌的发展 ,MMP 9和TIMP 1的平衡失调可能在肾癌的侵袭转移中发挥重要作用。

Expression and significance of matrix metalloproteinase-9 and its inhibitor in primary renal cell carcinoma

Objective To investigate the expression of matrix metalloproteinase-9 (MMP-9) and tissue inhibitor of metalloproteinase-1 (TIMP-1) in primary renal cell carcinoma (RCC) and their relationship with clinicopathological parameters.Methods The expression of MMP-9 and TIMP-1 in primary RCCs specimens were detected by immunohistochemical technique.Results The postive expression rate of MMP-9 and TIMP-1 in RCC and normal renal tissues was 63.63% and 10.00% (P<0.05),60.00% and 10.00% (P<0.05),respectively.Expression of MMP-9 was closely related to Robson stage,renal fibromembrane invasion and lymph node metastasis (P<0.05),but not related to pathological types of RCC (P>0.05).Expression of TIMP-1 was not correlated with clinicopathological parameters in RCC (P>0.05).Conclusion Up-regulation of MMP-9 expression may be involved in the progression of RCC.The imbalance between MMP-9 and TIMP-1 may play an important role in invasion and metastasis of RCC.