The toxic fraction of gliadin digests in coeliac disease. Isolation by chromatography on Biogel P-10 |
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Authors: | J Jos L Charbonnier J Mossé J P Olives M F de Tand J Rey |
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Affiliation: | 2. Laboratoire d''Etude des Protéines, Département de Physiologie et Biochimie Végétales, INRA, 78000 VersaillesFrance |
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Abstract: | Improvement in the fractionation of gliadin digests and in the isolation of toxic fractions was achieved using chromatography on Biogel P-10. Fraction V, one of the 11 fractions eluted from a peptic-tryptic digest of crude gliadin extracted from Cappelle wheat, significantly affected coeliac jejunal mucosa in organ culture. BV, gamma V, omega V, the corresponding fractions V from beta-, gamma- and omega-gliadins, displayed similar toxic effects. Fraction VI containing peptides with a lower molecular mass did not show any significant cytotoxic activity and, moreover, inhibited the toxicity of fraction V. Analysis of the toxic fractions V showed that they contained peptides of 7-8,000 molecular mass, rich in proline and glutamine and poor in aromatic amino acids and carbohydrates. Among the various fractions, V, beta V from beta-gliadin appeared the less heterogeneous. |
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Keywords: | Correspondence should be addressed to Pr.J. Jos Hôpital des Enfants-Malades 149 rue de Sèvres 75743 Paris Cédex 15 France. |
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