干扰素-β转染对胶质瘤的治疗和细胞因子的调节作用

目的 探讨干扰素-β（IFN-β）基因转染对胶质瘤的生长抑制和诱导凋亡的作用，明确IFN—B在SK—MG-1细胞系中对其他细胞因子的调节作用。方法 用IFN—β基因转染和蛋白刺激胶质瘤细胞系SKMG-1，通过MTT方法检测细胞增殖情况，并运用流式细胞技术检测其对凋亡的影响，同时用RT—PCR的方法检测细胞因子的表达变化。结果 IFN—β基因转染可以明显抑制细胞增殖，转染后48h和72h对肿瘤细胞的生长抑制率达到37．3％和46．0％。IFN—β基因转染还可以诱发细胞凋亡，转染后48h和72h肿瘤凋亡率达到31．7％和48．2％。RT—PCR结果显示在胶质瘤细胞系中IFN—β上调白细胞介素-1β（IL-1β）和肿瘤坏死因子-α（TNF—α）的mRNA表达，同时一过性下调白细胞介素-6（IL-6）的mRNA表达，而后在24h后上调IL-6的表达。结论 IFN—β基因转染可以抑制胶质瘤细胞系SK-MG-1的增殖，诱发细胞的凋亡，IFN—β对胶质瘤的生长抑制作用可能与其对其它细胞因子的调节作用有关。

The Effect of Interferon-β(IFN-β)Gene Transfection on Glioma and Cytokine Expressions

Objective To study the effect of interferon-β(IFN-β) on glioma cell line and cytokine expressions. Methods SK-MG-1l cells were stimulated with IFN-β protein and transfected with IFN-β liposome.MTT method and flow cytometry were applied to investigate the growth inhibition effect and apoptosis induction effect of IFN-βon SK-MG-1 cell line ,RT-PCR method was used to detect the cytokine expressions induced by IEN-β. Results IFN-βgene transfection can suppress the growth of SK-MG-1 cells and induce their apoptosis.We also found that interleukin-6(IL-6)) expression reduced transiently after IFN-βstimulation but interleukin-1β(IL-β)and tumor necrosis factor-α(TNF-α) expressions were upregulated by IFN-βtrasfection. Conclusion IFN-βcan suppress SK-MG-1 cell growth and induce apoptosis,which may be related to its regulation effect on cytokine expressions.