光镜及电镜下观察人胚涎腺肌上皮细胞

目的：在光镜及电镜下观察人胚涎腺肌上皮细胞的形态及组织结构。方法：选择１０例７至８月流产胎儿颌下腺，９例标本用石蜡包埋，ＨＥ及台兰染色光镜下观察。２例标本经环氧树脂包埋，在电镜下观察。结果：光镜下寺染色切片，在腺泡及闰管表面可清楚地看到扁平的肌上皮细胞。电镜观察，间以粒相连拉，胞浆内含有肌微丝，细胞吕不发达。结论：肌上皮细胞在胚胎期就已形成。ＨＥ染色，该细胞难以辨认，而用台盼兰染色观察该细胞简便易

OBSERVATION OF HUMAN EMBRYO SALIVARY GLAND MYOEPITHELIAL CELLS WITH LIGHT AND ELECTRON MICROSCOPE

Objective:To observe the morphology and histological structure of the human embryo salivary gland myoepithelial cells with light and electron microscope.Methods:Ten submandibular glands of miscarried embryos were included.Eight were embedded with paraffin,stained with HE and trypan blue,and observed by light microscope;Two were embedded with epoxy resin and observed with electron microscope.Results:Under the light microscope,myoepithelial cells were found around the acinus and the intercalated ducts.With Electron microscrope,the cells were joined by desmosomes,cytoplasm filled with actin filaments,and immature organelles.Conclusion:Myoepithelial cells appeared in embryo stage,but was difficult to be identified with HE stainning.It is a simple and convenient method to observe myoepithilial cells with trypan blue stained.The findings in electron microsgraph further conformed that the cells had epithelial and mesenchymal dual structure and feature.