| 细胞因子诱导不同来源的贴壁细胞分化为树突状细胞的研究 |
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| 引用本文： | 王月丹,谢玮,邱玉华,顾宗江,张学光.细胞因子诱导不同来源的贴壁细胞分化为树突状细胞的研究[J].现代免疫学,2000,20(3):140-144. |
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| 作者姓名： | 王月丹 谢玮 邱玉华 顾宗江 张学光 |
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| 作者单位： | 苏州医学院免疫学研究室,苏州,215007 |
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| 基金项目： | 国家自然科学杰出青年基金资助项目(No．39870825)及江苏省自然科学基金资助项目(BJ98100) |
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| 摘 要： | 通过贴壁的方法分离胚肝、脐带血、造血动员后的外周血及正常人外周血中的树突状细胞 (DC )的前体细胞 ,体外采用细胞因子诱导 ,并对其进行形态学观察和细胞表型分析 ;同时比较了不同来源DC的增殖、分泌IL 12的能力及激发同种异体T细胞和脐带血幼稚型T细胞 (naiveTcell)的作用。实验结果表明 ,经过造血动员后的外周血细胞产生的DC数量和纯度均较高 ,而且能经体外细胞因子诱导为功能性DC ,是较理想的DC来源
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| 关 键 词： | 树突状细胞 协同刺激分子 幼稚型T细胞 细胞因子 |
| 文章编号： | 1001-2478(2000)03-0140-05 |
| 修稿时间： | 1999-09-03 |
The Study of Dendritic Cells Induced by Cytokines from Different Adherent Cells in Vitro |
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| Wang Yuedan,Xie Wei,Qiu Yuhua,Gu Zongjiang,Zhang Xueguang.The Study of Dendritic Cells Induced by Cytokines from Different Adherent Cells in Vitro[J].Current Immunology,2000,20(3):140-144. |
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| Authors: | Wang Yuedan Xie Wei Qiu Yuhua Gu Zongjiang Zhang Xueguang |
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| Institution: | Wang Yuedan
,Xie Wei
,Qiu Yuhua
,Gu Zongjiang
,Zhang Xueguang
;(Department of Immunology, Suzhou Medical College, Suzhou 215007) |
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| Abstract: | The adherent precursors of dendritic cells (DC) isolated from fetal liver,cord blood,mobilized or nonmobilized peripheral blood were induced with cytokines to DC in vitro The growth of DC from different sources,and their ability to secrete IL12 and their potentials to stimulate the proliferation of naive T cells from cord blood were observed It was found that DC precursors from peripheral blood after mobilization were a good source of DC,and thus produced DC showed a high quantity of production and higher purity,and to be fully functional |
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| Keywords: | dendritic cell costimulatory molecule naive T cell cytokine |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
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