| 砷化物对HL-60细胞生长及基因组DNA甲基化的影响 |
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| 引用本文： | 卢光明,崔旭,黄福南,赵军平,丛涛,戚其平,姚孝远. 砷化物对HL-60细胞生长及基因组DNA甲基化的影响[J]. 中国地方病学杂志, 2001, 20(3): 161-163 |
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| 作者姓名： | 卢光明 崔旭 黄福南 赵军平 丛涛 戚其平 姚孝远 |
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| 作者单位： | 1. 解放军总医院基础所,
2. 中国预防医学科学院环监所, |
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| 基金项目： | 1999-2001年国家自然科学基金资助项目（38900123） |
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| 摘 要： | 目的：研究NaAsO2和 N3AO4cf HL-60细胞生长及基因组DNA甲基化的影响，方法：采用细胞培养和限制性内切酶解分析技术观察分析细胞生长及细胞基因组DNA甲基化水平。结果：（1）中无机砷化物对HL－60细胞生长具有抑制作用，特别是高浓度NaAsO2(2,10umol/L)和Na3AsO4(100,300umol/L)可明显地抑制HL－60细胞的生长（P＜0．01），随着砷化物浓度的增加，MspI酶解基因组DNA片段的分子量变化不大，但Hpa II酶解片段的分子量逐渐变大，结论：提示基因组DNA中CCGG核苷酸内侧C的甲基化总的水平有上升趋势。
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| 关 键 词： | 砷化物 细胞生长 脱氧核糖核酸甲基化 DNA 毒性 HL－60细胞 致癌物 |
| 文章编号： | 1000-4955(2001)03-0161-03 |
| 修稿时间： | 2000-11-02 |
Effects of arsenicals on growth and genomic DNA methylation in HL-60 cells |
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| LU Guang ming,CUI Xu,HUANG Fu nan,et al. Effects of arsenicals on growth and genomic DNA methylation in HL-60 cells[J]. Chinese Jouranl of Endemiology, 2001, 20(3): 161-163 |
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| Authors: | LU Guang ming CUI Xu HUANG Fu nan et al |
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| Abstract: | Objective To study the effects of arsenicals on growth and genomic DNA methylation of HL-60 cells.Methods Cell culture and methylation-sensitive restriction endonuclease digestion were used.Results ①Arsenicals caused inhibition obviously on HL-60 cells growth at the test concentrations,particularly in the groups of 2μmol/L,10μmol/L sodium arsenite,and the groups of 100 μmol/L,300 μmol/L sodium arsenate.②The length of HpaⅡrestriction fragments were substantially increased concurrently with the increasing of arsenic doses for treating the HL-60 cells,and no change was observed for MspI cutting bands.Conclusions Arsenicals can inhibit the growth of HL-60 cells,and cause hypermethylation in internal cytosine-methylation in the CmCGG sequence of the total genomic DNA. |
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| Keywords: | Arsenic Cell growth DNA methylation |
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