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Early detection of chemoradioresponse in esophageal carcinoma by 3'-deoxy-3'-3H-fluorothymidine using preclinical tumor models.
Authors:Smith Apisarnthanarax  Mian M Alauddin  Firas Mourtada  Hisanori Ariga  Uma Raju  Osama Mawlawi  Dongmei Han  William G Bornmann  Jaffer A Ajani  Luka Milas  Juri G Gelovani  K S Clifford Chao
Institution:Department of Radiation Oncology, The University of Texas M.D. Anderson Cancer Center, Houston, 77030, USA.
Abstract:PURPOSE: Early identification of esophageal cancer patients who are responding or resistant to combined chemoradiotherapy may lead to individualized therapeutic approaches and improved clinical outcomes. We assessed the ability of 3'-deoxy-3'-(18)F-fluorothymidine positron emission tomography (FLT-PET) to detect early changes in tumor proliferation after chemoradiotherapy in experimental models of esophageal carcinoma. EXPERIMENTAL DESIGN: The in vitro and ex vivo tumor uptake of (3)H]FLT in SEG-1 human esophageal adenocarcinoma cells were studied at various early time points after docetaxel plus irradiation and validated with conventional assessments of cellular proliferation thymidine (Thd) and Ki-67] and (18)F]FLT micro-PET imaging. Imaging-histologic correlation was determined by comparing spatial Ki-67 and (18)F]FLT distribution in autoradiographs. Comparison with fluorodeoxyglucose (FDG) was done in all experiments. RESULTS: In vitro (3)H]FLT and (3)H]Thd uptake rapidly decreased in SEG-1 cells 24 hours after docetaxel with a maximal reduction of over 5-fold (P = 0.005). The (3)H]FLT tumor-to-muscle uptake ratio in xenografts declined by 75% compared with baseline (P < 0.005) by 2 days after chemoradiotherapy, despite the lack of change in tumor size. In contrast, the decline of (3)H]FDG uptake was gradual and less pronounced. Tumor uptake of (3)H]FLT was more closely correlated with Ki-67 expression (r = 0.89, P < 0.001) than was (3)H]FDG (r = 0.39, P = 0.08). Micro-PET images depicted similar trends in reduction of (18)F]FLT and (18)F]FDG tumor uptake. Autoradiographs displayed spatial correlations between (18)F]FLT uptake and histologic Ki-67 distribution in preliminary studies. CONCLUSIONS: FLT-PET is suitable and more specific than FDG-PET for depicting early reductions in tumor proliferation that precede tumor size changes after chemoradiotherapy.
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