含驱白巴布期胶囊血清对人黑素瘤细胞A375增殖和迁移的影响

目的探讨驱白巴布期胶囊(QBC)对白癜风的治疗作用。方法大鼠ig给予QBC 0.54,2.7和5.4g·kg-1组,每天2次,连续3 d,末次给药2～3 h后腹主动脉采血,分离血清。用MTT比色法测定含QBZ血清体外对人黑素瘤A375细胞增殖的影响,酶学方法测定对酪氨酸酶活性的影响,比色法测定黑素含量,Transwell微孔膜法观察A375细胞迁移;显微镜下观察划痕后A375细胞伤痕愈合的能力。结果与正常对照组比较,给予QBC0.54 g·kg-1大鼠的30%含药血清,2.7 g·kg-1的10%含药血清和5.4 g·kg-1的20%含药血清均能促进A375细胞增殖。QBC 0.54 g·kg-1组的5%含药血清及QBC 5.4 g·kg-1组的5%,10%和20%含药血清均有促进酪氨酸酶活性的作用。QBC 2.7和5.4 g·kg-1组的20%含药血清具有显著促黑素含量增加的作用(P<0.01),且QBC5.4 g·kg-1含药血清促黑素含量增加的能力明显高于QBC 0.54和2.7 g·kg-1(P<0.05)。QBC 0.54,2.7和5.4 g·kg-1的20%含药血清均能显著提高A375细胞的迁移能力,且QBC 5.4 g·kg-1组促A375细胞迁移的能力明显高于QBC 0.54和2.7 g·kg-1组。划痕实验结果表明,QBC 0.54,2.7和5.4 g·kg-1组的含药血清均能明显促进A375细胞伤痕愈合,QBC 5.4 g·kg-1组促伤口愈合能力明显高于QBC 0.54和2.7 g·kg-1。结论 QBC可促进黑素细胞增殖,提高酪氨酸酶活性,促进黑素细胞迁移,对白癜风可能具有一定的治疗作用。

Effects of Qubaibabuqi capsule containing serum on proliferation and migration of human melanoma A375 cells

OBJECTIVE To explore the effect of treating vitiligo with Qubaibabuqi capsule(QBC).METHODS Rats were randomly divided into normal control,QBC 0.54,2.7 and 5.4 g·kg-1 after 12 h fasting diet.Rats in QBC groups were ig given QBC 0.54,2.7 and 5.4 g·kg-1,twice daily,for 3 d.Rats in normal control group were given an equal volume of 0.5% sodium carboxymethyl cellulose.At 2-3 h after the last administration,the blood samples were collected through the abdominal aortia and serum was seperated.A375 melanoma cells were cultured with QBC containing serum in vitro.Proliferation of A375 cells was deterimined by MTT,tyrosinase activity was measured by zymological method,melanin was detected by colorimetric,motility was assessed using the Transwell micropore filter method,and the ability of A375 melanoma cells to heal wound was observed under a microscope.RESULTS Compared with normal control,30% QBC contained serum of QBC 0.54 g·kg-1,10% QBC contained serum of 2.7 g·kg-1 and 20% QBC contained serum of 5.4 g·kg-1 significantly enhanced A375 cell proliferation.5% QBC contained serum of QBC 0.54 g·kg-1,and 5%,10% and 20% QBC contained serum of 5.4 g·kg-1 could remarkably stimulate the tyrosinase of A375 melanoma cells.20% QBC contained serum of QBC 2.7 and 5.4 g·kg-1 could increase the melanin content significantly,and this effect of QBC 5.4 g·kg-1 was better than that of 0.54 and 2.7 g·kg-1.20% QBC contained serum of QBC 0.54,2.7 and 5.4 g·kg-1 obviously enhanced melanin synthesis.QBC 5.4 g·kg-1 promoted the migration of A375 cells better than 0.54 and 2.7 g·kg-1.The scratch test results showed that QBC 0.54,2.7 and 5.4 g·kg-1 significantly promoted wound healing of A375 cells,and QBC 5.4 g·kg-1 more effective than QBC 0.54 and 2.7 g·kg-1.CONCLUSION QBC can promote melanocyte proliferation,increase tyrosinase activity,promote migration of melanocytes and may contribute to the treatment of vitiligo.