intermedin对单侧输尿管梗阻大鼠肾间质纤维化的影响

目的 观察intermedin( IMD)对单侧输尿管梗阻(UUO)大鼠肾间质纤维化的影响.方法 雄性Wistar大鼠40只,按随机数字表法分为假手术组(n=10,行左输尿管分离术)、模型组(UUO,n=10)、氯沙坦组(n=10)和IMD组(n=10),后3组行左输尿管结扎术.各组大鼠分别于术后第14、21天随机选取5只,腹主动脉采血并留取梗阻侧肾组织之后处死.HE、Masson染色观察肾组织病理变化；比色法测定血尿素氮(BUN)、血肌酐(Scr)及新鲜肾组织羟脯氨酸(Pro)含量；免疫组化方法检测肾组织中转化生长因子β1 (TGF-β1)、IMD的表达水平,并进行半定量分析.结果 与假手术组相比,不同时间点UUO组血BUN、Scr、肾组织Pro含量及TGF-β1、IMD的阳性表达均显著升高(P＜0.05)；与UUO组相比,氯沙坦组血BUN、Scr、肾组织Pro含量及TGF-β1、IMD表达均降低(P＜0.05),IMD组除IMD表达增加外,其余均降低(P＜0.05).结论 IMD可减轻UUO肾间质纤维化,改善肾功能,其机制可能与拮抗纤维化炎性介质TGF-β1有关.

Effect of intermedin on renal interstitial fibrosis of unilateral ureteral obstruction rats

Objective To observe the effect of intermedin on renal interstitial fibrosis of unilateral ureteral obstruction rats. Methods Forty male Wistar rats were randomly divided into two groups: the sham-operation group (n=10) underwent the left ureteral dissection, the other 30 rats were made as unilateral ureteral obstruction models and divided into the model group (UUO), the losartan group, the IMD group (each group n=10). At the day 14, 21 after operation, randomly 5 rats from each group were blooded by abdominal arotic and obstructive kidneys were taken out. The renal histopathological changes were observed by HE and Masson staining.The BUN, Scr, and hydroxyproline (Pro) of the obstructive kideys were measured by colorimetry. The expression of TGF-β1 and intermedin was observed by immunohistochemisty staining. Results Compared with the sham-operated group, the levels of BUN, Scr, Pro, TGF-β1 and IMD in the model group increased (P<0.05). Compared with the UUO group, the levels of Scr, BUN, Pro, TGF-β1 and IMD in the losartan group were lower (P<0.05). However, IMD in the IMD group was significantly up-regulated(P< 0.05), the others were down-regulated (P<0.05). Conclusion IMD can improve the renal interstitial fibrosis, and the mechanism maybe antagonizes the TGF-β1.