| 安徽药菊耐盐突变体的筛选 |
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| 引用本文： | 薛建平张爱民,高翔,盛玮.安徽药菊耐盐突变体的筛选[J].中国中药杂志,2004,29(9):834-837. |
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| 作者姓名： | 薛建平张爱民 高翔 盛玮 |
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| 作者单位： | 淮北煤炭师范学院,生物系,安徽,淮北,235000 |
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| 摘 要： | 目的 :通过组织培养技术和EMS诱导突变相结合筛选出愈伤组织耐盐突变系。方法 :取药菊叶片诱导形成愈伤组织 ,用 0.2%和 0.5%EMS分别经浸渍法和滴加法处理 ,培养 15d后逐次转移到含NaCl 0.5% ,1.0%和 1.5%的筛选培养基上 ,进而获得耐盐愈伤组织突变体。结果与结论 :经耐盐性和耐盐稳定性检验 ,证明所获得的愈伤组织是突变体。
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| 关 键 词： | 药用菊花 甲基磺酸乙酯 诱变 耐盐突变体 |
| 文章编号： | 1001-5302(2004)09-0834-04 |
| 收稿时间： | 2003/12/18 0:00:00 |
Selection of sodium chloride tolerant mutants in Chrysanthemum morifolium in Anhui |
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| XUE Jian-ping;ZHANG Ai-ming;Gao Xiang;SHENG Wei.Selection of sodium chloride tolerant mutants in Chrysanthemum morifolium in Anhui[J].China Journal of Chinese Materia Medica,2004,29(9):834-837. |
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| Authors: | XUE Jian-ping;ZHANG Ai-ming;Gao Xiang;SHENG Wei |
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| Institution: | Department of Biology, Huaibei Coal Industry Teachers' College, Huaibei 235000, China. xuejp2000@yahoo.com.cn |
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| Abstract: | Objective: To select the sodium chloride t ol erant mutants of the Chrysanthemum morifolium callus through tissue culture and EMS (ethylmethane sulfonate) treatment. Method: Ca lli were induced form the leaves of C.morifolium. The calli were treated wit h 0.2% and 0.5% EMS, respectively. After 15 days'culture, the calli were transp lanted to selection media with 0.5%,1.0% and 1.5% NaCl, and the sodium chloride tolerant mutants were selected out. Result and Conclusion: After tested the sodium chloride tolerant stability, the callus selected are found to be the mutants indeed. |
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| Keywords: | Chrysanthemum morifolium EMS inductio n NaCl tolerant mutants |
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