高渗盐溶液对失血性休克大鼠红细胞膜黏弹性的影响

采用微管吸吮技术，观察高渗盐溶液对失血性休克大鼠红细胞膜黏弹性的影响。Wistar大鼠随机分为0.9%NaCl(NS)、7．5％ NaCl(HS)、5％ NaCl-3.5% NaAc(HSA)三组。10min内放血使平均动脉压降至5．3kPa，维持90min。休克模型完成后，分别按4ml／kg体重静脉注入NS、HS、HSA，5min内输完。取血测定休克前、后及给药后的红细胞膜黏弹性。结果表明，休克后红细胞膜弹性模量、黏性系数较休克前显著增加。治疗后，HS组与NS组比较，弹性模量增加，黏性系数显著降低；HSA组与NS、HS组比较，弹性模量和黏性系数显著降低。结论 是HSA可显著改善失血性休克大鼠红细胞膜黏弹性。

Effect of Hypertonic Saline Solution on the Viscoelasticities of Erythrocyte Membrane in Rats Subjected to Hemorrhagic Shock

We have studied the effect of hypertonic saline solution on the viscoelasticities of erythrocyte membrane in hemorrhage shocked rats using micropippette aspiration technique. Wistar rats were randomly divided into three groups of 0.9% NaCl(NS),7.5% NaCl(HS) and 5% NaCl 3.5% NaAc(HSA),respectively. The animals were bled to reach a mean arterial pressure of 5.3 kPa in 10 minutes and maintained in shock for 90 minutes. 4 ml/kg NS,HS and HSA was given intravenously and respectively in 5 minutes following hemorrhagic shock. The blood was collected to determine the viscoelasticities of erythrocyte membrane at baseline, shock and after treatment. The results showed that the elastic moduli and viscous coefficients of erythrocyte membrane were increased obviously following hemorrhagic shock. HS raised elastic moduli and reduced viscous coefficients significantly compared with NS after treatment. The elastic moduli and viscous coefficients of erythrocyte membrane were decreased remarkably in HSA group than in NS and HS group.These data suggested that HSA could improve the viscoelasticities of erythrocyte membrane significantly in rats subjected to hemorrhagic shock.