| 应用基因表达谱芯片技术研究膦甲酸钠处Jurkat细胞后的差异表达基因 |
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| 引用本文： | 刘妍,成军,王刚,陆荫英,王建军,张喜全,王祥建. 应用基因表达谱芯片技术研究膦甲酸钠处Jurkat细胞后的差异表达基因[J]. 解放军医学杂志, 2004, 29(5): 386-388 |
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| 作者姓名： | 刘妍 成军 王刚 陆荫英 王建军 张喜全 王祥建 |
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| 作者单位： | 100039,北京,解放军第302医院传染病研究所基因治疗研究中心;江苏省连云港市正大天晴制药有限公司药物研究所 |
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| 基金项目： | 军队回国留学人员启动基金(编号98H038)、国家自然科学基金攻关项目(编号C030114020,30070689)、军队"十五"科技攻关青年基金项目(编号01Q138)和军队"十五"科技攻关面上项目(编号01B135)资助课题. |
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| 摘 要： | 目的 筛选膦甲酸钠处理人T淋巴细胞系Jurkat细胞后的差异表达基因。方法 应用基因表达谱芯片技术对膦甲酸钠处理的Jurkat细胞和以生理盐水处理的相同细胞的mRNA进行检测。结果 Jurkat细胞经膦甲酸钠处理后,所检测的1152条目的基因中,有94条产生差异表达,其中38条基因表达增强,56条基因表达降低。结论 应用基因表达谱芯片成功筛选了膦甲酸钠处理淋巴细胞后差异表达基因,为进一步阐明膦甲酸钠的免疫调节机制及深人了解膦甲酸钠用于治疗病毒性肝炎的药理作用机制提供依据。
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| 关 键 词： | 膦甲酸钠 免疫调节 基因表达谱芯片 |
| 修稿时间： | 2003-04-08 |
Study on differentially expressed genes in Jurkat cells treated with phosphonoformate using DNA microarray |
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| Liu Yan,Cheng Jun,Wang Gang et al. Gene Therapy Research Center. Study on differentially expressed genes in Jurkat cells treated with phosphonoformate using DNA microarray[J]. Medical Journal of Chinese People's Liberation Army, 2004, 29(5): 386-388 |
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| Authors: | Liu Yan Cheng Jun Wang Gang et al. Gene Therapy Research Center |
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| Affiliation: | Liu Yan,Cheng Jun,Wang Gang et al. Gene Therapy Research Center,Institute of Infectious Diseases,302 Hospital of PLA,Beijing 100039,China |
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| Abstract: | Objective To screen the genes differentially expressed in gene expression in human lymphoma cell line Jurkat cells treated with phosphonoformate (PFA). Methods cDNA microarray technique was employed to detect the mRNA expressed in Jurkat cells treated with PFA or 0.9 percent sodium chloride, respectively. Results Among 1 152 genes there were 94 genes with different expression, of which 38 genes were upregulated and 56 genes were downregulated in Jurkat cells treated with PFA compared with those treated with 0.9 percent sodium chloride. Conclusions cDNA microarray technique was successfully used to screen the genes with different expression in Jurkat cells treated with PFA, and the results brought some new clues for the study of the immune regulation mechanism of PFA. |
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| Keywords: | phosphonoformate immune regulation cDNA microarray |
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