富含半胱氨酸的酸性分泌蛋白在人大肠癌、大肠腺瘤的表达及其临床意义

目的]探讨富含半胱氨酸的酸性分泌蛋白(SPARC)在人大肠癌、大肠腺瘤中的表达及其临床意义。方法]采用免疫组化SABC法检测65例人大肠癌、32例人大肠腺瘤及29例正常人大肠组织中SPARC的表达。结果]免疫组化结果显示SPARC在人大肠腺癌细胞及间质细胞均有不同程度表达。大肠癌、大肠腺瘤及正常组织三者之间比较,SPARC在组织腺管细胞中的阳性表达率差异无统计学意义(P0.05),而在间质细胞中的表达率差异显著(P0.05)。另外,在人大肠癌间质细胞中,SPARC的阳性表达率与癌组织分化程度、淋巴结转移与否呈明显相关性(P0.05),而与患者性别、年龄、肿瘤部位无关(P0.05)。结论]SPARC有可能作为评估大肠肿瘤浸润转移及其预后的生物学指标,为进一步研究大肠肿瘤的早期筛查与诊断提供了新的思路与方法。

Relationship between expression of SPARC and its clinical significance in human colorectal carcinoma and colorectal adenoma

Objective]To investigate the expression and clinical significance of secreted proteinacidic and rich in cysteine（SPARC） in human colorectal carcinoma and colorectal adenoma. Methods]Using the im- munohistochemical technique to investigate the expression of SPARC in 65 cases colorecta carcinoma, 32 cases colorectal adenoma and 29 cases normal colorectal tissues. Results] Immunohistochemical results show that the expression of SPARG were different in large intestinal cancer cells and stromal cells. Compared with colon cancer, colonic adenoma and normal tissue, the positive expression rates of SPARC in the gland cells have no significant differences （P 〉 0.05）, while the difference was clear in stro- mal cells（P〈0.05）. Besides, the expression of SPARC in colorectal cancer stromal cells is significantly cor- related with the degree of differentiation and degree of lymphatic metastasis, but not with patient＇s factors such as age,gender and the part of the tumor. Conclusion]SPARC is not only can be used as a biological indicator for estimating invasion,metastasis and the prognosis of colonic carcinoma,but also can provides a new idea and method for the further study of colon cancer screening and early diagnosis.