| 大孔树脂纯化丹参总酚酸的研究 |
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| 引用本文： | 赵萱,傅超美,徐晓秋. 大孔树脂纯化丹参总酚酸的研究[J]. 中药与临床, 2014, 0(1): 17-20 |
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| 作者姓名： | 赵萱 傅超美 徐晓秋 |
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| 作者单位： | 成都中医药大学药学院 |
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| 摘 要： | 目的:确立大孔树脂纯化丹参总酚酸的最佳纯化工艺。方法:以静态吸附率和解吸率为考察指标,确定大孔树脂型号,并确定纯化工艺参数。结果:选择HPD-400型号大孔树脂用于丹参总酚酸的纯化工艺,最佳吸附工艺条件为:上样吸附流速为2BV/h,上柱液的浓度为0.5 g生药/mL,pH值为4;解析前,以3BV的水洗脱除杂,最佳解析工艺条件为:选择乙醇为洗脱剂,洗脱剂浓度为40%,pH值为5,控制解析流速为3BV/h。结论:所建立的方法简便、准确,可用于丹参总酚酸的纯化。
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| 关 键 词： | 丹参总酚酸 大孔树脂 纯化工艺 |
Purification of Total phenolic acid of Danshen by macroporous resin |
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| ZHA,Xuan,FU Chao-mei,XU Xiao-qiu. Purification of Total phenolic acid of Danshen by macroporous resin[J]. Pharmacy and Clinics of Chinese Materia Medica, 2014, 0(1): 17-20 |
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| Authors: | ZHA Xuan FU Chao-mei XU Xiao-qiu |
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| Affiliation: | (Pharmaey College, Chengdu University of Traditional Chinese Medicine;The Ministry of Education Key Laboratory of Standardization of Chinese Herbal Medicine;State Key Laboratory Breeding Base of Systematic Research, Development and Utilization of Chinese Medicine Resources, Chengdu 611137, China) |
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| Abstract: | Objective： To establish the purification technology of Total phenolic acid of Danshen by macroporous resin. Method： Static adsorption rate and desorption rate were taken as indexs to optimize the type of macroporous resin and purification technology parameters. Result： The HPD-400 macroporous resin was chosen in the purification technology of Total phenolic acid of Danshen. Optimum adsorption technological conditions were as following： The adsorption rate was 2BV/h. The concentration was 0.5 g raw drug/mL with pH of 4.3BV water was used to elute impurity before desorption. Optimum desorption technological conditions were as following： Eluted with 40% ethanol aqueous at the flow rate 3BV/h, and pH was 5. Conclusion： The method is simple, accurate, specific and can be applied in the purification of Total phenolic acid of Danshen. |
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| Keywords: | Total phenolic acid of Danshen macroporous resin purification technology |
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