间二硝基苯对大鼠肝脏氧化损伤的研究

目的研究间二硝基苯（ｍ－ＤＮＢ）对肝脏的损伤并探讨其中毒机制。方法采用整体与离体实验相结合的方式对大鼠进行ｍ－ＤＮＢ染毒，观察大鼠肝脏脂质过氧化情况。结果随染毒剂量的增加和染毒时间的延长，染毒组大鼠肝脏丙二醛（ＭＤＡ）含量明显增加，呈良好的剂量－效应关系和时间－效应关系；低剂量、短时间染毒时，谷胱甘肽（ＧＳＨ）含量增加，大剂量、长时间染毒时，其含量降低；氧化型谷胱甘肽（ＧＳＳＧ）及ＧＳＳＧ／ＧＳＨ比值均随染毒剂量和时间的增加而增加；肝细胞的超氧阴离子自由基（Ｏ－２·）、羟自由基（·ＯＨ）的产生量明显增加。结论诱发脂质过氧化及产生自由基是ｍ－ＤＮＢ的主要中毒机制之一。

The study on the oxidative damage induced by m DNB to rat hepatocyte

Objective The study was designed to examine the damage induced by m DNB to hepatocytes. Methods Two ways of exposure to m DNB were carried out:(1)rats were administered in vivo with different doses of m DNB;(2)cultured rat hepatocytes were exposed to different concentrations of m DNB.Then the changes of indices in lipid peroxidation were observed. Results The contents of MDA increased significantly in a manner dependent on exposure time and concentration of m DNB ;the contents of GSH increased as a response to a stress for short period of exposure and at lower doses,but decreased for longer period of exposure and at higher doses;the contents of GSSG and especially the ratio of GSSG/GSH increased with the rise of doses;the production of hydroxy free radicals and superoxide radicals were elevated obviously. Conclusion The study demonstrated that the lipid peroxidation might play an important role in m DNB toxicity.