| 呼吸道合胞病毒对人肺腺癌细胞系PAa的影响 |
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| 引用本文： | 陈杭薇,钱桂生,邬光惠,李继成.呼吸道合胞病毒对人肺腺癌细胞系PAa的影响[J].第三军医大学学报,2000,22(3):213-215. |
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| 作者姓名： | 陈杭薇 钱桂生 邬光惠 李继成 |
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| 作者单位： | 1. 第三军医大学附属新桥医院全军呼吸内科研究所,重庆,400037
2. 北京军区总医院,北京,100700 |
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| 基金项目： | 全军“九五”医药卫生科研基金!重点资助项目 (98Z0 61 ) |
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| 摘 要： | 目的：探讨呼吸道合胞病毒（ＲＳＶ）感染对体外培养人肺腺癌细胞系ＰＡａ的影响。方法：应用ＲＳＶ体外感染ＰＡａ细胞，观察ＰＡａ细胞的变化：聚合酶链反应（ＰＣＲ）结合单链构象多态性分析（ＳＳＣＰ）检测ＰＡａ细胞ｐ５３基因突变情况。结果：ＲＳＶ感染ＰＡａ细胞２４ｈ后，细胞出现融合病变，形成巨融合细胞；病变细胞ｐ５３第５、６、７、８、９外显子突变热点区域未见单链多态性变化。结论：ＲＳＶ对ＰＡａ细胞形态影响明
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| 关 键 词： | 呼吸道合胞病毒 肺腺癌 pAa细胞 SSCP |
Effects of respiratory syncytial virus on human lung adenocarcinoma cell line PAa and p53 gene mutation |
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| CHEN Hang-wei,QIAN Gui-sheng,WU Guang-hui,LI Ji-cheng.Effects of respiratory syncytial virus on human lung adenocarcinoma cell line PAa and p53 gene mutation[J].Acta Academiae Medicinae Militaris Tertiae,2000,22(3):213-215. |
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| Authors: | CHEN Hang-wei QIAN Gui-sheng WU Guang-hui LI Ji-cheng |
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| Abstract: | Objective: To study the effects of respiratory syncytial virus (RSV) on the morphology of human lung adenocarcinoma cell line PAa and the mutation of p53 Methods:PAa cells were infected with RSV and the mutation of p53 in PAa cells was observed with polymerase chain reaction single strand conformation polymorphism (PCR SSCP) Results: 24 hours after RSV infection, huge fused PAa cells were formed PCR SSCP revealed no change of exon 5, 6, 7, 8 and 9 of p53 gene in the fused PAa cells Conclusion: RSV infection affects significantly the morphology of PAa cells but its effects on the changes of genes in PAa cells needs further investigation |
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| Keywords: | respiratory syncytial virus tumor cell p53 gene PCR SSCP |
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