| TRAIL基因与鸡贫血病毒vp3基因体外协同效应的研究 |
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| 引用本文： | 彭冬君,尹燕华,王宇哲,孙军,宗义强,屈伸.TRAIL基因与鸡贫血病毒vp3基因体外协同效应的研究[J].医学分子生物学杂志,2004,1(2):94-97. |
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| 作者姓名： | 彭冬君 尹燕华 王宇哲 孙军 宗义强 屈伸 |
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| 作者单位： | 华中科技大学同济医学院基础医学院生物化学与分子生物学系,武汉市,430030;华中科技大学同济医学院基础医学院生物化学与分子生物学系,武汉市,430030;华中科技大学同济医学院基础医学院生物化学与分子生物学系,武汉市,430030;华中科技大学同济医学院基础医学院生物化学与分子生物学系,武汉市,430030;华中科技大学同济医学院基础医学院生物化学与分子生物学系,武汉市,430030;华中科技大学同济医学院基础医学院生物化学与分子生物学系,武汉市,430030 |
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| 摘 要： | 目的研究TRAIL基因与鸡贫血病毒vp3基因在体外协同诱导肝癌细胞系HepG2凋亡的作用。方法从人外周血淋巴细胞总RNA中扩增出TRAIL基因的cDNA序列,构建含TRAIL基因的真核表达重组体,与鸡贫血病毒vp3基因在体外共转染HepG2细胞,研究其协同诱导HepG2细胞凋亡作用。结果 单独转染TRAIL基因与vp3基因均可诱导HepG2细胞凋亡;将两种基因共转染HepG2细胞,发现其凋亡率明显增加。结论TRAIL基因与vp3基因在诱导HepG2细胞凋亡中存在正协同效应。
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| 关 键 词： | 肝脏肿瘤 凋亡 TRAIL基因 vp3基因 |
| 修稿时间： | 2004年3月9日 |
Hepatoma Cell Apoptosis-inducing Effect of TRAIL Cooperates with vp3 In Vitro |
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| PENG Dongjun,YIN Yanhua,WANG Yuzhe,SUN Jun,ZONG Yiqiang,QU Shen.Hepatoma Cell Apoptosis-inducing Effect of TRAIL Cooperates with vp3 In Vitro[J].Journal of Medical Molecular Biology,2004,1(2):94-97. |
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| Authors: | PENG Dongjun YIN Yanhua WANG Yuzhe SUN Jun ZONG Yiqiang QU Shen |
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| Institution: | PENG Dongjun,YIN Yanhua,WANG Yuzhe,SUN Jun,ZONG Yiqiang,QU Shen
Department of Biochemistry and Molecular Biology,School of Basic Medical Sciences,Tongji Medical
College,Huazhong University of Science and Technology,Wuhan,430030,China |
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| Abstract: | Objective To explore the hepatoma cell line HepG2 apoptosis-induced effect of TRAIL cooperating with vp3 in vitro. Methods Eukaryotic expression vector for TRAIL cDNA acquired from human lymphocytes was constructed. After co-transfecting with vp3 gene, HepG2 apoptosis-induced effect was observed. Results TRAIL and vp3 gene could induce apoptosis of HepG2 cells in vitro, and enhanced effect was observed by co-transfecting TRAIL with vp3 gene. Conclusion Positive-cooperative effect of TRAIL and vp3 exist in apoptosis-induction of HepG2 cells. |
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| Keywords: | liver neoplasms apoptosis tumor necrosis factor-related apoptosis inducing ligand vp3 gene |
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