急性心肌梗死患者IL-6和IL-10水平测定及其临床意义

目的：分析急性、陈旧性心肌梗死和急性心肌梗死溶栓前后炎症因子白细胞介素—6(IL-6)和抗炎因子白细胞介素—10(IL-10)含量的变化并探讨其临床意义。方法：用ELISA分析试剂盒检测24例急性心肌梗死和20例陈旧性心肌梗死患血浆IL-6和IL-10含量。10例急性心肌梗死患行溶栓治疗，分别检测治疗前后IL-6和IL-10的含量。结果：急性和陈旧性心肌梗死组血浆IL-6和IL-10含量均显高于健康对照组。急性心肌梗死组IL-6和IL-10含量较陈旧性心肌梗死组显增高。急性心肌梗死患溶栓后血浆IL-10的含量明显降低，IL-6的含量有所增高。急性心肌梗死组IL-10的含量与肌酸激酶(CK)和肌酸激酶—同功酶(CK—MB)均呈不同程度的显正相关。IL-6的含量与CK和CK—MB无显相关。结论：急性心肌梗死患血浆IL-6和IL-10均显增高，它们在缺血损伤和再灌注过程中可能存在相互调节的关系。

Clinical Significance of Plasma Levels of IL-6 and IL-10 in Patients with Acute Myocardial Infarction

Objective: To study the variation of plasma inflammatory cytokine IL-6 and antiinflammatory cy-tokine IL-10 in patients with acute and old myocardial infarction, patients with acute myocardial infarction before and after thrombolytic therapy. Methods: 24 patients with acute myocardial infarction (10 patients subjected to thrombolytic therapy) and 20 patients with old myocardial infarction were studied. Plasma levels of IL-6 ang IL-10 were detected by ELISA. Results: In this study, significantly increased level of IL-6 and IL-10 were observed in patients with acute and old myocardial infarction. Plasma levels of LL-6 and IL-10 were significantly higher in acute myocardial infarction group compared with old myocardial infarction group. IL-10 levels were obviously decreased and EL-6 level were slightly increased in patients with acute myocardial infarction after thrombolytic therapy. From the statistic analysis, we have demonstrated that there were significant positive correlation between IL-10 and crea-tine kinade(CK), IL-10 and creatine kinade-MB(CK-MB), respectively. Conclusion: Our studies demonstrated increased plasma levels of DL-1 and EL-10 in patients with acute myocardial infarction. They could probably regulate each other in myocardial ischemia and reperfusion.