富氢水对放射性皮肤损伤的作用研究

目的 观察富氢水对放射性皮肤损伤的作用。方法 利用Varian直线加速器发射6MeV电子线照射大鼠后臀部皮肤制作损伤模型，治疗组予富氢水喷雾创面，对照组予生理盐水、持续4周，对比创面愈合情况，计算创面愈合率；MDA（丙二醛）、SOD（超氧化物歧化酶）试剂盒测定创面组织MDA含量和SOD活力，ELISA法测定血清IL-6和EGF含量。结果 单次照射44Gy的6MeV电子线，成功建立动物模型。治疗后与生理盐水组相比，富氢水组创面愈合明显增快，创面组织MDA含量低、SOD活力高；血清IL-6含量较低、EGF含量高。结论 富氢水能够促进放射性皮肤损伤的愈合，其作用机制与氧化损伤和炎性反应有关。

Effect of Hydrogen-rich Water on Skin Radiation Injury in Rat Models

Objective To study the effect of hydrogen-rich water on skin radiation injury. Methods The skin radiation injury models on rats were established by locally irradiating with electron linear accelerator. Experimental group was applicated of hydrogen-rich water spray and control group was applicated of NS against rays after the skin ulcer for 4 weeks. The wound healing time was observed and wound healing rate were calculated. MDA content and SOD activity in wound tissue and IL-6 and EGF content in the blood were assessed by MDA, SOD assay kit and ELISA assay. Results The model of acute radiation-induced skin ulcers in rats was established with 44 Gy electron linear to the skin. The wound healing time of the hydrogen-rich water group was significantly shorter than the NS group. In the hydrogen-rich water group, the MDA content and IL-6 content were lower, and SOD activity and EGF levels were significantly higher than the NS group after 1,2,3,4 weeks after administration. Conclusion Hydrogen-rich water can relieve oxidative damage and inflammation, which may be related to the positive effect of hydrogen-rich water in skin radiation injury.