两种破坏粘膜的方法对原位膀胱癌模型建立的影响

目的比较两种不同的膀胱粘膜损伤方法对细胞移植法原位膀胱癌模型建立的影响及优缺点。方法4～6周龄裸鼠两组，每组10只。麻醉后经尿道置入膀胱静脉留置针，导尿后一组注入100ul10％胰酶保留30min，另一组注入100ul0．1mol·L^-1Hcl15s．PBS冲洗后将浓度为1×10^7L^-1L。的人膀胱癌T24细胞悬液注入两组鼠膀胱保留1～2h。另设一组5只导尿冲洗后直接注入细胞保留1～2h。定期观察裸鼠一般情况。28d后断颈处死，解剖观察膀胱成瘤及转移情况，并取膀胱及可疑肾脏行病理学检查。结果胰酶破坏组到2w有2只间断出现淡红色血尿，下腹肿块不明显。到28d后处死，肉眼2只成瘤，约占膀胱50％以上。病理检查证实成瘤2只，多局限于粘膜，未突破肌层。盐酸破坏组约12d开始出现血尿，其中4只可及下腹肿块，2只于2d死亡。到28d处死．共8只大体成瘤，2只见右肾肿大。病理检查示8只成瘤，细胞结构紊乱，未突破浆膜层，两只肾转移。未行粘膜破坏组无明显变化，也无瘤形成。结论胰酶破坏膀胱粘膜对动物损伤轻，但成瘤率低；盐酸破坏粘膜对动物损伤稍大，但成瘤率满意，在细胞移植法建立原位膀胱癌模型可明显提高成瘤率。

The impact of two different Methods of bladder mucosal injury in situ cell transplantation in animal model of bladder cancer

Objective Comparing the impact of two different Methods of bladder mucosal injury in situ cell transplantation in animal model of bladder cancer, and evaluating their advantages and disadvantages.Methods Two groups of nude mice with 10 mice in each group were anaesthetized Intraperitoneally and then the indwelling intravenous needles were put through the urethra to the mice bladder under sterile conditions. After catheterization one group mice were injected 100 ul 10% trypsin into bladder and retained 30 rain,another group were injected 100ul 0.1mol.L-1 Hcl and retained 15 s.Then the two groups of nude mice were perfused 1 × 10^7L^-1 in human bladder cancer T24 cell suspension into the bladder to retain 1-2h after washing twice with PBS. Separately another group of five post-catheterization nude mice were directly perfused the cells into bladder to retain the same 1-2h. Regularlly observation of the general growth of nude mice. each group of nude mice were sacrificed and anatomised after 4 weeks, observed of bladder tumor formation and metastasis, and to take bladder tissue and suspicious line of renal pathological examination,Results In the Trypsin damaged group there were two underwent reddish intermittent hematuria and lower abdominal mass was not obvious. To 28 clays more the mice were sacrificed, generally two mice suffered bladder tumor visibly and Pathologically, mostly limited to mucosa, muscular layer did not break through,accounting for more than 50%, of bladder. Hydrochloric acid damage group of mice began to undergo intermittent haematuria about 12 days, 4 of which can only reach the lower abdomen mung bean size of mass, mobilely touched. There are two deaths in 2days.To 28 days off the mice were sacrificed, a total of eight with bladder tumor formation in general and Pathologically,Cell structure disorder,no breakthrough in serosal layer, there are two with visible swelling of the right kidney, the surface uneven, color dark red,and the transferred cancer Pathologically of the two kidneys.The group of no Mucosal damage were no significant change.Conclusions Trypsin destruction of the bladder mucosa is slight, but the tumor formation rate is lower, Destruction of mucosal injury by hydrochloride is serious, but the tumor formation rate of satisfaction, in the cell transplantation model established by in situ bladder cancer,it can increase tumor formation rate greatly.