舒宁康水提液抗过敏药效研究

目的:观察舒宁康水提液对肥大细胞脱颗粒及组胺释放的影响,研究其抗过敏作用.方法:以卵蛋白(OVA)为致敏原,在OVA质量浓度为40 mg·L-1作用30 min条件下,观察舒宁康0.1,1,10 g·L-1在体外对致敏大鼠肥大细胞脱颗粒及其释放组胺量的影响.结果:以色甘酸钠为阳性对照,舒宁康水提液具有明显的抑制肥大细胞脱颗粒作用,模型组、舒宁康水提液高、中、低剂量组及阳性对照组脱颗粒率分别为(47.53±5.57)％,(25.11±2.56)％,(35.71±6.99)％,(47.11±4.26)％,(22.87±6.52)％;舒宁康水提液对组胺的释放亦有显著的抑制作用,与模型组比,舒宁康水提液高、中、低组及阳性对照组的抑制率分别为16.85％,20.06％,6.58％,16.85％.结论:舒宁康水提液有良好的抗过敏作用,为将其开发成抗敏中药提供了实验依据.

Antiallergic Effect of Aqueous Extract from Shuningkang

Objective:To study the antiallergic action of Shuningkang by investigating the effect of aqueous extract from Shuningkang on mast cell degranulation and histamin releasing in vitro by PMC degranulation percentage and histamine releasing assay. Method:OVA was used as allergen. In the concentration of 40 mg·L^-1,30 min conditions, the influences of aqueous extract from Shuningkang 0.1,1,10 g·L^-1on sensitized rat mast cell degranulation and histamine release were investigated. Result:The extract of Shuningkang could inhibit rat PMC degranulation and histamine releasing in vitro. PMC degranulation rates in model group,high, middle, low does groups of Shuningkang and Cromoglicate Sodium group were (47.53±5.57)%,(25.11±2.56)%,(35.71±6.99)%,(47.11±4.26)% and(22.87±6.52)% respectively. Shuningkang showed significant inhibition on histamine release, compared with the model group. In the high, middle, low does groups of Shuningkang and Cromoglicate Sodium group, the inhibition rates on histamine release were 16.85%,20.06%,6.58%,16.85%. Conclusion:Shuningkang has certain effect on skin allergies.