银杏叶聚戊烯醇同系物体外抑制乳腺癌MCF-7细胞株增殖作用及机制研究

目的:研究银杏叶聚戊烯醇同系物体外抑制MCF-7细胞增殖的作用并初步探讨作用机制.方法:经制备反相高效液(RT-HPLC)获得银杏叶聚戊烯醇同系物,MTT法检测其对MCF-7细胞增殖的影响,选取抑制细胞增殖作用强的同系物P3,用流式细胞仪检测5,10,20 mg·L-1 P3对MCF-7细胞周期、细胞凋亡的影响.结果:经提取分离获得10个银杏叶聚戊烯醇同系物,依次命名为P1,P2,P3……P10,其中P3对MCF-7细胞株增殖的抑制作用最强,IC50为10.32 mg·L-1,其作用机制低浓度时以阻滞细胞周期于G2/M期为主,高浓度可诱导凋亡.结论:银杏叶聚戊烯醇同系物P3为银杏叶提取物抑制MCF-7增殖的活性成分,其作用机制与阻滞细胞周期,诱导细胞凋亡有关.

Growth-inhibiting Effect and its Molecular Mechanism of Polyprenols from ginkgo biloba on Breast Cancer Cells MCF-7 in vitro

Objective:To study the growth-inhibiting effect of polyprenols from Ginkgo biloba on breast cancer cells MCF-7 in vitro and the related molecular mechanism.Method:Polyprenols were extracted by preparative Reversed-phase high-performance liguid chromatography(RP-HPLC).The growth-inhibiting effect of polyprenols from Ginkgo biloba on breast cancer cells MCF-7 was evaluated in vitro.The polyprenols with best antiproliferative effect was screened and figured out.The influence of polyprenol P3(5,10,20 mg · L-1) on mitotic cycle and apoptosis of MCF-7 cells was analyzed with flow cytometry.Result:Ten polyprenol monomers were obtained by preparative RP-HPLC,and were orderly named P1,P2,P3……P10.The best effective growth-inhibiting on MCF-7 cells was P3 with IC50 of 10.32 mg · L-1.At low concentration,its mechanism appeared to be delaying on G2/M of mitotic cycle,and at high concentration to be inducing cell apoptosis.Conclusion:Polyprenol P3 from Ginkgo biloba could inhibit growth of breast cancer MCF-7 cells in vitro.Its molecular mechanism is linked with delaying on mitotic phase(G2/M) and inducing cell apoptosis.