子宫内膜癌hMSH2及P53蛋白表达的意义

目的]探讨错配修复蛋白hMSH2及抑癌基因蛋白P53表达在子宫内膜癌发生中的意义。方法]用免疫组织化学SP法检测92例子宫内膜癌、12例子宫内膜增生过长及6例正常子宫内膜中hMSH2蛋白及P53蛋白的表达情况,采用秩和检验和χ2检验及确切概率法统计分析。结果]hMSH2蛋白在子宫内膜癌、子宫内膜增生过长和正常子宫内膜组织的阳性表达率分别为63.04%、25.00%和33.33%,统计学差异显著(P<0.05);P53蛋白在子宫内膜癌和子宫内膜增生过长阳性表达率45.65%和50.00%,高于正常子宫内膜组织的16.67%,但3组间无显著差异(P>0.05)。hMSH2蛋白表达阳性与阴性子宫内膜癌组,P53蛋白阳性表达率分别为65.90%与26.47%,两种蛋白表达呈正相关(P<0.05)。结论]碱基错配增多导致的基因突变积累可能是子宫内膜癌的发病原因。

Expressions of hMSH2 and P53 proteins in endometrial carcinoma

Objective] To study the expressions and their significance of DNA Mismatch Repair Gene hMSH2 protein and tumour suppressor gene p53 protein in endometrial carcinoma. Method] SP immunohistochemistry was used to detect the expressions of hMSH2 and P53 protein in 92 cases of endometrial carcinoma (EC), 12 cases of endometrial hyperplasia (EH) and 6 cases of normal endometrium (NE). Results] The positive expression rates of hMSH2 protein in EC group was 63.04%, significantly higher than that in EH and NE groups which were 25.00% and 33.33% respectively(P<0.05). The positive expression rates of P53 protein in EC and EH groups were 45.65% and 50.00%, higher than that in NE being 16.67%, but there was no significant difference among them (P>0.05). The expression of hMSH2 protein was positively related to the expression of P53 protein in EC cases (P<0.05). Conclusion] Mutation that due to bases mismatch increasing may be a reason of endometrial carcinoma.